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[ Hot attention of chemical machinery equipment network ] Real-time fluorescence PCR (realtime fluorescence PCR) is to add a substrate containing a fluorescent group to the PCR system, and realize the real-time detection of the fluorescent signal of each cycle product in the amplification reaction.
Template quantitative and qualitative analysis methods
.
Chemical machinery and equipment network hotspots focus on chemical machinery and equipmentTemplate quantitative and qualitative analysis methods
.
Real-time PCR (Real-time PCR) is one of the nucleic acid detection tools commonly used in clinical practice
.
However, the number of targets detectable per reaction is limited by current models
.
A major limitation is the limited number of target genes that can be detected in a single reaction
.
According to the number of detection channels of the current mainstream fluorescent PCR instruments, the number of target genes that can be detected by a single reaction is difficult to exceed 4-6, which limits the application of this technology to complex diseases involving multiple targets
.
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However, the number of targets detectable per reaction is limited by current models
.
A major limitation is the limited number of target genes that can be detected in a single reaction
.
According to the number of detection channels of the current mainstream fluorescent PCR instruments, the number of target genes that can be detected by a single reaction is difficult to exceed 4-6, which limits the application of this technology to complex diseases involving multiple targets
.
Li Qingge's team from the School of Life Sciences, Xiamen University reported a new fluorescent PCR technology called "MeltArray", which improved the single-tube detection capability of fluorescent PCR to more than an order of magnitude, and systematically demonstrated the technology through multiple clinical application scenarios Powerful and flexible detection capabilities
.
A related paper was published online in the Proceedings of the National Academy of Sciences (PNAS) on February 24, 2022
.
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A related paper was published online in the Proceedings of the National Academy of Sciences (PNAS) on February 24, 2022
.
The research team found that the MeltArray assay can identify 10 times more targets in current real-time PCR assays without the need for additional enzymes or separate reactions
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The research team explored the use of MeltArray in various contexts and successfully achieved a 20-plex assay of human Y chromosome microdeletions, identified Escherichia coli serovars, a 24-plex assay that simultaneously identifies and quantifies respiratory pathogens, and a Feasibility of mutational analysis
.
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The research team explored the use of MeltArray in various contexts and successfully achieved a 20-plex assay of human Y chromosome microdeletions, identified Escherichia coli serovars, a 24-plex assay that simultaneously identifies and quantifies respiratory pathogens, and a Feasibility of mutational analysis
.
The MeltArray method has the advantages of diversity, versatility, high degree of automation, and short turnaround time.
It not only fills the technical gap between low-level PCR and high-throughput detection for a long time, but also greatly improves fluorescent PCR.
instrument detection capability
.
It is foreseeable that MeltArray will have very broad prospects in clinical application
.
It not only fills the technical gap between low-level PCR and high-throughput detection for a long time, but also greatly improves fluorescent PCR.
instrument detection capability
.
It is foreseeable that MeltArray will have very broad prospects in clinical application
.
Original title: Xiamen University has developed a high-level multiplex fluorescent PCR detection technology, which improves the PCR detection ability by more than an order of magnitude
