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Assessment of Magnaporthe grisea mating type by spore PCR
Time of Update: 2021-01-30
grisea conidia directly for PCR analysis without extraction of DNA.
This spore PCR protocol can be applicable to other fungi and may be useful for rapid PCR assay without DNA preparation in analyzing transformants and field collections.
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A novel method of growing fungi for DNA extraction
Time of Update: 2021-01-30
In this note, the possibility of extracting DNA suitable forPCR amplification from mycelium grown on reverse agar media is documented.
Miniprep DNA (b-d), ITS 1-4 amplification (e-g) and HinfI restriction digests (h-j) from fungi grown on malt extract reverse agar.
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Directed Evolution of Enzymes: How to Improve Nucleic Acid Polymerases for Biocatalysis
Time of Update: 2021-01-30
In this chapter, we introduce basic techniques necessary for improving enzyme function by directed evolution, and we use the “tuning” of bacteriophage T7 RNA polymerase toward decreased fidelity as an example.
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Magnetic Particle-Based Separation Techniques for Monitoring Bacteria from Natural Environments
Time of Update: 2021-01-30
Physical separation of either intact target cells or specific molecules from many environments can result in a suspension free of contaminating particles, nontarget cells and biological inhibitors and highly enriched in the target cells or molecules of interest.
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Rapid Sequencing of Parasite rRNA
Time of Update: 2021-01-30
Owing to the presence of a series of highly conserved domains, rRNAs can serve as general indicators, even among the most distant living species ( 6 – 11 ).
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The Impact o Molecular Techniques on the Study of Meningococcal Disease
Time of Update: 2021-01-30
This antigenically variable gram-negative diplococcus has the potential, however, to cause rapidly progressing meningitis and fulminant septicemia, either separately or together ( 1 , 2 ).
Once present in the bloodstream, meningococci grow rapidly and their highly toxic lipo-oligosaccharides frequently cause extensive tissue damage and severe toxic shock.
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Nature sub-journal: Eat more vegetables, intestinal bacteria will be healthier
Time of Update: 2021-01-29
, researchers from the University of Trento in Italy, King's College London and the Harvard School of Public Health published a long-term follow-up article in Nature Medicine entitled "Microbiome connections with host metabolism and habitual diet 1,098 deeply phenotyped individuals", which found that eating plant-based foods and healthy eating habits encourages the presence of beneficial microbes in the gut and reduces the risk of diseases such as obesity and diabetes.
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Probiotic market size of nearly 100 billion Coto bio-with star strains to create the future
Time of Update: 2021-01-29
, the global probiotic market will be worth about 40 billion euros (about 300 billion yuan) in 2019, according to the World Health Agency. China's probiotic market is growing at an average annual rat
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Endurance exercise is good for gut bacteria: reduce inflammation-promoting bacteria without changing diet
Time of Update: 2021-01-29
study lead author Eveliina Munukka said: "Recent studies have shown that exercise improves the gut microbiome.
effects on heart metabolic health the researchers also found that phospholipids and cholesterol in VLDL (very low density lipoprotein) particles decreased with exercise.
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Study on the domestication of oxygen tolerance of Probio-M8
Time of Update: 2021-01-29
recommended in an article in the China Food Journal, "The Study of Oxygen Resistance domestication of Probio-M8 of Breast Bifido" This issue introduces the research team of Professor Zhang Peace of
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Use good probiotics to keep your intestines open
Time of Update: 2021-01-29
To days, whether people work overtime or play with mobile phones and so on, staying up late has become a common meal, coupled with nervous anxiety, external stimulation, three meals from time to time, high-fat high-protein diet, so that the rest of the diet is irregular, resulting in gastrointestinal health emergency.
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What about high blood lipids? - Cheesecella Zhang to help
Time of Update: 2021-01-29
Blood sample analysis After 30 days of a high-fat diet, TC, TG, and LDL-C levels in rats in the hyperlipidemia and probiotic groups were found to increase significantly compared to the control group, and HDL-C levels decreased significantly (P TG and LDL-C levels in the serum decreased by 31.3% and 30.8% (P Total Cholesterol (TC), Triglycerides (TG), HDL-C, and LDL Cholesterol (LDL-C) 2.
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Zhang Peace Team: Probiotics enhance the nerve stimulation activity of the intestinal bacteria to relieve stress and anxiety
Time of Update: 2021-01-29
(1) Subjects were subjected to oral plant Lactobacillus P-8 (n-43) or placebo (n-36) for 12 weeks; (2) after intervention, the diversity of fecal flora decreased in the placebo group, while there was
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Transformation of Mycoplasmas
Time of Update: 2021-01-27
Transformation of mycoplasmas was not clearly demonstrated until 1987 when Dybvig and Cassell were able to show the introductionof the Gram-positive transposon Tn 916 intoAcholeplasma laidlawii andMycoplasma pulmonis ( 1 ).
This was the first direct evidence that mycoplasmas could be transformed under laboratory conditions.
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Intracellular Multiplication of Legionella Species and the Influence of Amoebae on Their Intracellular Growth in Human Monocytes: Mono Mac 6 Cells and Acanthamoeba castellanii as Suitable In Vitro Models
Time of Update: 2021-01-27
Legionella pneumophila (predominantly serogroup 1) is detected in most cases of legionellosis; other species only occasionally cause infections, predominantly in immunocompromized patients ( 1 – 4 ).
Legionellae are present in the water in these systems, within the amoebae, flagellates, and ciliates in which they replicate ( 2 , 5 , 6 ).
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Impact of Molecular Methods on Clinical Bacteriology
Time of Update: 2021-01-27
Are patients and the infections from which they may be suffering managed more effectively and efficiently through the application of molecular methods?
Detailed theoretical and practical guidance on the application of these techniques to the diagnosis, management, and epidemiology of a wide range of infections is provided in the succeeding chapters.
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Electrophysiological Characterization of Bacterial Pore-Forming Proteins in Planar Lipid Bilayers
Time of Update: 2021-01-27
Together with patch-clamp, the planar lipid bilayer technique is one of the electrophysiological approaches used to study the biophysical properties of bacterial pore-forming proteins.
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Streaking Bacteria for Single Colonies
Time of Update: 2021-01-27
1. Initial inoculum: - From solid media, touch an isolated colonies with a sterile applicator or toothpick.
- From liquid media, touch the culture with the end of a sterile applicator.
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Bacteriophage Plaques: Theory and Analysis
Time of Update: 2021-01-27
These two environments may be distinguished in terms of their spatial structure, i.e., the degree to which they limit diffusion, motility, and environmental mixing.
The study of phage growth within spatially structured environments, such as that seen during phage plaque formation, is important for three reasons.
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Sensitive and Rapid Detection of Cholera Toxin-Producing Vibrio cholerae Using Loop-Mediated Isothermal Amplification
Time of Update: 2021-01-27
The LAMP assay requires 12–18 min for amplification with a single colony on selective agar from cholera toxin (CT)-producingVibrio cholerae strains and less than 60 min with human feces and seafood samples.
