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    Home > Biochemistry News > Microbiology News > Transformation of Mycoplasmas

    Transformation of Mycoplasmas

    • Last Update: 2021-01-27
    • Source: Internet
    • Author: User
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    Transformation of mycoplasmas was not clearly demonstrated until 1987 when Dybvig and Cassell were able to show the introductionof the Gram-positive transposon Tn
    916
    into
    Acholeplasma laidlawii
    and
    Mycoplasma pulmonis
    (
    1
    ). This was the first direct evidence that mycoplasmas could be transformed under laboratory conditions. These studies haveled to numerous attempts to develop mutagenesis schemes (
    2

    4
    ), as well as cloning vectors for gene analysis and other functions in mycoplasmas (
    5

    8
    ). The three common methods to transform bacteria, chemical treatment with calcium chloride or polyethylene glycol (PEG),liposome-mediated delivery of encapsulated
    DNA
    , and electroporation-based procedures have all been used in mycoplasmas andwill be described here.
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