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Shandong draws a road map for the upgrade of fertilizer and coal chemical industry
Time of Update: 2022-02-21
The plan emphasizes that it is necessary to use advanced coal gasification technology to transform the existing fixed-bed gasification equipment, build a batch of clean gasification furnaces, and encourage enterprises to extend the industrial chain through the comprehensive utilization of coal-to-synthetic gas.
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Epitope Mapping by Differential Chemical Modification of Antigens
Time of Update: 2020-12-25
Because of the large size of a typical contact epitope in an antigen-antibody crystal, it is unlikely for an antibody to bind exclusively to a contiguous stretch of the polypeptide chain and not also to contact residues apart in sequence, but close in space.
One such method is the differential chemical modification of the free and antibody-bound protein.
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Epitope Mapping by Chemical Fragmentation
Time of Update: 2020-11-29
The use of antigen fragments generated by specific chemical cleavage is a relatively simple “library” approach for epitope mapping in which overlapping fragments are screened with antibody on Western blots.
The fragments on Western blots can often be recognized unequivocally usingM r s predicted from the sequence.
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Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map ProteinProtein Interactions
Time of Update: 2020-11-14
Here, we describe the methods for capturing intermediates in the import process through the manipulation of the energy state of chloroplasts, and the use of two different chemical cross-linking approaches to examine the molecular interactions that mediate the import process and to assess the assembly state of the translocons.
