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    • Stable Isotope Labeling by Amino Acids in Cell Culture for Quantitative Proteomics
      Time of Update: 2020-12-29
      Stable isotope labeling by amino acids in cell culture (SILAC), one of the more widely used methodsfor quantitative proteomics, is a metabolic-labeling strategy that encodes whole cellular proteomes. Incorporation of the “heavy” amino acid occurs through cell growth, protein synthesis, and turnover.

    • Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS
      Time of Update: 2020-11-16
      Plant metabolic pathways and the molecular and atomic fluxes through them can be deduced using stable isotopically labeled substrates. Here, we summarize the application of gas chromatography-time of flight mass spectrometry (GC-TOF-MS) for metabolic studies using differently labeled elemental isotopes applied to both intact organelles and whole plant tissue.
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