-
Determination of the molecular weight of DNA-binding proteins using UV-crosslinking and SDS-PAGE
Time of Update: 2021-03-01
We describe the use of UV-crosslinking in combination with SDS -PAGE to determine the approximate molecular weight ofDNA -binding proteins.
Following irradiation with a UV light source, the DNA/protein complex is subjected to SDS-PAGE and its molecular weight determined by comparison with appropriate protein standards.
-
Purification of Bacteriophages and SDS-PAGE Analysis of Phage Structural Proteins from Ghost Particles
Time of Update: 2021-02-13
These particles should be preferred to the entire bacteriophages for one-dimensionalSDS -PAGE analysis of phage structural proteins, since running of the phage proteins through the gel is not disturbed by the presence of the phage DNA.
-
SDS-PAGE Separation of Proteins Produced by Beans During Development
Time of Update: 2020-11-22
Proteins produced during the process of seed germination in beans will be separated using SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE in this laboratory).
The proteins will be separated on a polyacrylamide gel matrix.
-
Analysis of Rice Proteins Using SDS-PAGE Shotgun Proteomics
Time of Update: 2020-11-19
In this chapter we describe the workflow used in our laboratory to analyze rice leaf samples using label-free shotgun proteomics based onSDS -PAGE fractionation of proteins.
Rice proteomics has benefitted substantially from successful execution of shotgun proteomics techniques.
-
Determination of protein molecular weight (SDS-polyacrylamide gel electrophoresis)
Time of Update: 2020-10-30
Therefore, the migration rate of various SDS-protein complexes in electrophoresis is no longer affected by the original charge and shape, but is separated by the molecular sieve effect of the gel according to the size of the molecule, and its effective migration rate is linearly related to the log of molecular weight.
-
The SDS-polyacrylamide gel electrophoresis method measures the relative molecular weight of proteins.
Time of Update: 2020-10-29
18.15 Tris (trihydroxy methamethane), plus about 80 ml of heavy steamed water, with 1mol/LHCl to pH to 8.8, diluted with heavy steamed water to the final volume of 100 ml, 4 degrees C refrigerator storage.
-
SDS-PAGE, protein transfer, western Blot reagent preparation.
Time of Update: 2020-10-20
5. Hiculation , save at room temperature Note: the solution should be cooled to room temperature and then adjust the pH, because the pH of tris solution varies greatly with the temperature, the temperature increases by 1oC, the pH of the solution is reduced by about 0.03 units.
(see kara company Catalog-N1) 1 mol/L Tris (PH6.8) 1.