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A Novel Approach Using MALDI-TOF/TOF Mass Spectrometry and Prestructured Sample Supports
Time of Update: 2020-12-17
Mass spectrometry (MS)-based proteomic profiling and protein identification has become a powerful tool for the discovery of new disease biomarkers.
Here, we present a strategy using MALDI-TOF/TOF MS to analyze fractionated human serum samples for proteomic profiling and then identify serum peptides from these proteomic profiles.
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MALDI-ToF MS Analysis of Glycosyltransferase Activities on Gold Surface Arrays
Time of Update: 2020-12-14
In this chapter, we describe the preparation of SAMs-coated gold surface arrays presenting carbohydrate or (glyco)peptide substrates, either pre-formed or directly synthesized on-chip, and MALDI-ToF MS analysis of glycosyltransferase activities on these immobilized substrates.
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SPR/MS: Recovery from Sensorchips for Protein Identification by MALDI-TOF Mass Spectrometry
Time of Update: 2020-12-08
Surface plasmon resonance is widely used to study binding interactions with proteins, potentially yielding information on kinetics, thermodynamics and active concentrations.
Mass spectrometry, on the other hand, can be used for specific identification of proteins in amounts comparable to the levels that can be captured on a Biacore SPR sensorchip.
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Protein Identification by MALDI-TOF Mass Spectrometry
Time of Update: 2020-11-23
MALDI-TOF mass spectrometers are now commonplace and their relative ease of use means that most non-specialist labs can readily access the technology for the rapid and sensitive analysis of biomolecules.
One of the main uses of MALDI-TOF-MS is in the identification of proteins, by peptide mass fingerprinting (PMF).
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Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS
Time of Update: 2020-11-09
Using OLIMP, we can harness the selective power of a specific wall hydrolase together with the speed and sensitivity of mass spectrometry to provide highly reproducible structural and compositional information about the wall molecule of interest.
