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Research reveals new functions of Golgi membrane protein PAQR11 on lipid metabolism and immune regulation
Time of Update: 2021-03-23
by PAQR11 via altering protein stability of phosphodiesterase 4D by Chen Yan’s research group. Protein stability affects lipolysis, thereby regulating the body's obesity symptoms.
Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex Subunit Proteins From Higher Plants
Time of Update: 2021-03-13
The OEC subunit proteins are dissociated from the PSII-enriched membranes by alkaline treatment or salt treatment, and then purified by ion-exchange chromatography using an automated high-performance liquid chromatography system.
One- and Two-Dimensional Blue Native-PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes
Time of Update: 2021-03-12
Blue native polyacrylamide gel electrophoresis (BN-PAGE) is a powerful method for separating protein complexes from biological membranes under native conditions.
Protein Extraction and Gel-Based Separation Methods to Analyze Responses to Pathogens in Carnation (Dianthus caryophyllus L)
Time of Update: 2021-03-12
It is clear that the protocols for the first stages of a standard proteomics workflow must be optimized to each biological system and objectives of the research.
Phenol Extraction of Proteins for Proteomic Studies of Recalcitrant Plant Tissues
Time of Update: 2021-03-10
It allows efficient protein recovery and removes nonprotein components in the case of plant tissues rich in polysaccharides, lipids, and phenolic compounds.
Total Protein Extraction with TCA-Acetone
Time of Update: 2021-03-08
We describe a procedure allowing extraction of total proteins that performs efficiently with a large variety of plant tissues, based on simultaneous precipitation and denaturation with TCA and 2ME in cold acetone.
On-Chip Detection of Low-Molecular-Weight Recombinant Proteins in Plant Crude Extracts by SELDI-TOF MS
Time of Update: 2021-03-07
A complementary protocol is then presented to generate a standard curve with the SELDI system, allowing the protein to be quantified in different transgenic lines.
Laurdan Studies of Membrane Lipid-Nicotinic Acetylcholine Receptor Protein Interactions
Time of Update: 2021-03-04
The extrinsic fluorescent probe Laurdan (6-dodecanoyl-2-dimethylamino naphthalene) exhibits extreme sensitivity to the polarity and to the molecular dynamics of the dipoles in its environment. Dipolar relaxation processes are reflected
Electron Crystallography of Membrane Proteins
Time of Update: 2021-03-02
However, sample preparations that sandwich the two-dimensional crystals between symmetrical carbon films reduce the charge-induced specimen drift, and the flatness of the preparations can be optimized by the choice of the grid material and the preparation protocol.
Membrane Insertion of Small Proteins
Time of Update: 2021-02-27
The insertion of the protein into proteoliposomes can be followed by protease digestion and Western blot analysis.
Analysis of Bacterial Outer Membrane Proteins
Time of Update: 2021-02-16
The cell envelope of Gram-negative bacteria comprises an inner and an outer membrane separated by a layer of peptidoglycan (Fig. 1 ).
Use of a Yeast-Based Membrane Protein Expression Technology to Overexpress Drug Resistance Efflux Pumps
Time of Update: 2021-02-14
We have developed a heterologous membrane protein expression system that can be used to study the structure and function of these proteins in the non-pathogenic, genetically stable, and versatile eukaryotic model organism,Saccharomyces cerevisiae .
Scientists in the United States have discovered a new way to extract antibacterial proteins from eggs
Time of Update: 2021-02-05
the purity of the ferro-delivery protein extracted from citric acid and ammonium sulfate exceeded 85% and the recovery rate was more than 83%.
Lalormande develops meaty, high-protein yeast extract
Time of Update: 2021-02-02
Wozniak the company hopes meat processing will be a major application in the food industry as Prime Lyfe 175A the world.
Assembly of Bacterial Outer Membrane Proteins
Time of Update: 2021-02-01
Various methods that are routinely used to study the subcellular localization of membrane proteins in wild-type Gram-negative bacteria fall short in genetic studies addressing the biogenesis of outer membrane proteins (OMPs).
Preparation of Blotted Membrane for Protein Microsequencing
Time of Update: 2021-01-31
This chapter aims to provide a step-by-step procedure in preparing protein samples of limited quantities for partial sequence analysis.
Extraction of Cell Wall-Bound Teichoic Acids and Surface Proteins from Listeria monocytogenes
Time of Update: 2021-01-30
In this chapter, we describe methods for the extraction and analysis of (i) WTAs, (ii) covalently, and (iii) non-covalently cell wall-bound surface proteins from the Gram-positive pathogenListeria monocytogenes .
Analysis of Interactions Between Viral Replicase Proteins and Plant Intracellular Membranes
Time of Update: 2021-01-19
Because many plant viruses replicate in association with the endoplasmic reticulum (ER), we also discuss two techniques to specifically analyze the interaction of viral proteins with these membranes.
Membrane and Protein Dynamics in Virus-Infected Plant Cells
Time of Update: 2021-01-16
Therefore, to meet the challenges of diverse cell biology studies that will be derived from ongoing functional genomics projects, a series of methods relevant to the characterization of membrane and protein dynamics in virus-infected cells are provided here.