2.2 g high-speed high-resolution miniaturized double photon fluorescence microscope is present.

After more than 3 years of concerted efforts, Peking University, together with the Chinese PLA Academy of Military Medical Sciences, formed an interdisciplinary team to successfully develop a new generation of high-speed high-resolution miniaturized dual photon fluorescence microscope, weighing only 2.2 grams.
team used the microscope to obtain clear, stable images of brain neurons and synapses in mice during free behavior.
original paper was published online May 29 in nature's sub-journal, Nature Methods.
a press conference on the morning of May 31st, Cheng peace academician gave a detailed introduction.
A new generation of miniaturized twin photon fluorescence microscopes, which are small in size and weigh only 2.2 grams, are suitable for wearing on the craniofacial windows of small animal heads and record dynamic signals of dozens of neurons and thousands of synapses in real time.
in large animals, it is also expected to achieve long-range observation of multi-probe wearing and multi-skull windows with different brain regions.
compared to single photon excitation, double photon excitation has good optical fault, deeper bio-tissue penetration and other advantages, its horizontal resolution of 0.65 m, imaging quality and commercialization of large-scale desktop dual photon fluorescence microscope can be comparable, far better than the current field-led, the core team of the United States Brain Science Program developed miniaturized wide-field microscope.
uses dual-axis symmetrical high-speed micro-electromechanical system transverse scanning technology, imaging frame rate has reached 40Hz (256 x 256 pixels), with multi-area random scanning and 10,000 lines per second line scanning capacity.
In addition, the system enables the effective use of micro-dual photonic microscopes for the most widely used fluorescent probes in the field of brain science, such as GCaMP6, using a photonic crystal fiber designed to conduct 920nm fibre-second lasers.
the use of flexible fiber beam for the reception of fluorescent signals, to solve the animal's activities and behavior due to fluorescent transmission fiber optic cable drag and drop the problem.
, combined with photogenetics technology, is expected to precisely manipulate the activity of neurons and neural circuits while structural and functional imaging.
Miniaturized double photon fluorescence microimaging has changed the way cell and subcellular structures are observed in freely active animals, and can be used to observe multi-scale, multi-level dynamic changes such as synapses, neurons, neural networks, remotely connected brain regions, etc. under natural behavior conditions such as animal foraging, lactation, platform jumping, fighting, playing, sleep, or before, during and after learning.
China Science Journal reporter learned that the results in the end of 2016 the United States Annual Conference of Neuroscience, May 2017 cold spring port Asian brain science conference, including a number of Nobel Laureates at home and abroad neuroscientists highly praised.
"By any standard, this microscope represents a major technological invention that will change the way we observe cell and subcellular structures in free-flowing animals," Professor Alcino J Silva of the University of California, Los Angeles, wrote in a commentary.
opens the door to neurons and bark imaging.
Systemic Neurobiology is entering a new era of imaging observations of complex biological events in identifiable cell and subcellular structures in cell populations to gain a deeper understanding of the core engineering principles of evolutionary brain loops for complex behavior.
there is no doubt that this extraordinary invention has taken us one step closer to that goal.
, it is reported that at present, the research and development team is leading the construction of "multi-modal cross-scale biomedical imaging" 13th national major scientific and technological infrastructure, and actively participate in the upcoming launch of China's brain science program.
can be expected that the miniaturization of the dual photon fluorescence microimaging system will play an indispensable role in achieving the strategic goal of "analyzing the brain, understanding the brain, imitating the brain".
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