A metagenomic assembly approach to assess biome-specific gene sets

Microbiome [IF:165] ① On the basis of two commonly used metagenomic assembly methods, single-sample assembly and multi-sample mixed assembly, a mixed assembly method (Mix-assembly) was develop. The core is to cluster the genes obtained by the first two methods to obtain mixed Redundant gene sets;② The mixed method is significantly better than the other two methods in the number of non-redundant genes, complete genes and annotable functional genes;③ The low abundance genes in the mixed gene set are mainly derived from multi-sample mixing, The high-abundance genes were derived from single-sample assembly; (4) Reanalysis of the Baltic Sea environmental samples using the mixed-assembly method expanded the original gene set by a factor of 10, greatly expanding the information available in the sampl. Evaluating metagenomic assembly approaches for biome-specific gene catalogues05-06, doi: 11186/s40168-022-01259-2[Editor's comment] Currently, metagenomic reads are assembled into contigs, single-sample assembly and multi-sample mixing (co- assembly) are two commonly used assembly methods, both of which have potential advantages and disadvantag. Recently, Microbiome published the latest resear. Based on these two assembly methods, the author developed a mixed assembly method (Mix-assembly), the core of which is to cluster the genes obtained by the two methods to obtain a mixed non-redundant gene s. Further reanalysis of Baltic environmental samples using the method resulted in a 10-fold expansion of the original gene s. In conclusion, this study shows that the hybrid assembly method may be a feasible method to increase the information of metagenomic samples, which deserves attention and attemp.   Disclaimer: This article only represents the author's personal opinion and has nothing to do with China Probiotics Netwo. Its originality and the text and content stated in the text have not been verified by this site, and this site does not make any guarantee or commitment to the authenticity, completeness and timeliness of this text and all or part of its content and te. Readers are only for reference and please Verify the relevant content yourse. Copyright Notice Some articles reproduced on this site are not original, and their copyright and responsibility belong to the original auth. All reprinted articles, links and pictures on this website are for the purpose of conveying more information, and the source and author are clearly indicat. Media or individuals who do not wish to be reprinted can contact us for infringing information that can provide sufficient eviden. , bio149 will be deleted within 12 hours after confirmati. Users are welcome to submit original articles to 86371366@. com, which will be published on the homepage after review, and the copyright and responsibility of the articles belong to the send.

Microbiome① On the basis of two commonly used metagenomic assembly methods, single-sample assembly and multi-sample mixed assembly, a mixed assembly method (Mix-assembly) was develop.

The core is to cluster the genes obtained by the first two methods to obtain mixed Redundant gene sets;② The mixed method is significantly better than the other two methods in the number of non-redundant genes, complete genes and annotable functional genes;③ The low abundance genes in the mixed gene set are mainly derived from multi-sample mixing, The high-abundance genes were derived from single-sample assembly; (4) Reanalysis of the Baltic Sea environmental samples using the mixed-assembly method expanded the original gene set by a factor of 10, greatly expanding the information available in the sampl.

Evaluating metagenomic assembly approaches for biome-specific gene catalogues05-06, doi: 11186/s40168-022-01259-2[Editor's comment] Currently, metagenomic reads are assembled into contigs, single-sample assembly and multi-sample mixing (co- assembly) are two commonly used assembly methods, both of which have potential advantages and disadvantag.

Recently, Microbiome published the latest resear.

Based on these two assembly methods, the author developed a mixed assembly method (Mix-assembly), the core of which is to cluster the genes obtained by the two methods to obtain a mixed non-redundant gene s.

Further reanalysis of Baltic environmental samples using the method resulted in a 10-fold expansion of the original gene s.

In conclusion, this study shows that the hybrid assembly method may be a feasible method to increase the information of metagenomic samples, which deserves attention and attemp.

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Microbiome① On the basis of two commonly used metagenomic assembly methods, single-sample assembly and multi-sample mixed assembly, a mixed assembly method (Mix-assembly) was develop.

The core is to cluster the genes obtained by the first two methods to obtain mixed Redundant gene sets;② The mixed method is significantly better than the other two methods in the number of non-redundant genes, complete genes and annotable functional genes;③ The low abundance genes in the mixed gene set are mainly derived from multi-sample mixing, The high-abundance genes were derived from single-sample assembly; (4) Reanalysis of the Baltic Sea environmental samples using the mixed-assembly method expanded the original gene set by a factor of 10, greatly expanding the information available in the sampl.

Evaluating metagenomic assembly approaches for biome-specific gene catalogues05-06, doi: 11186/s40168-022-01259-2[Editor's comment] Currently, metagenomic reads are assembled into contigs, single-sample assembly and multi-sample mixing (co- assembly) are two commonly used assembly methods, both of which have potential advantages and disadvantag.

Recently, Microbiome published the latest resear.

Based on these two assembly methods, the author developed a mixed assembly method (Mix-assembly), the core of which is to cluster the genes obtained by the two methods to obtain a mixed non-redundant gene s.

Further reanalysis of Baltic environmental samples using the method resulted in a 10-fold expansion of the original gene s.

In conclusion, this study shows that the hybrid assembly method may be a feasible method to increase the information of metagenomic samples, which deserves attention and attemp.

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Microbiome[IF:165]① Based on the two commonly used metagenomic assembly methods, single-sample assembly and multi-sample mixing, a mixed assembly method (Mix-assembly) has been develop.

The core is to combine the genes obtained by the first two metho.

class, and obtained a mixed non-redundant gene set;② the mixed method was significantly better than the other two methods in terms of the number of non-redundant genes, complete genes and annotable functional genes;③ the low-abundance genes in the mixed gene set were mainly derived from Multi-sample mashup, while high-abundance genes were derived from single-sample assembly;④ Reanalysis of Baltic environmental samples using the mashup method expanded the original gene set 10-fold, greatly expanding the information available in the samp.

Evaluating metagenomic assembly approaches for biome-specific gene catalogues05-06, doi: 11186/s40168-022-01259-2[Editor's comment] Currently, metagenomic reads are assembled into contigs, single-sample assembly and multi-sample mixing (co- assembly) are two commonly used assembly methods, both of which have potential advantages and disadvantag.

Recently, Microbiome published the latest resear.

Based on these two assembly methods, the author developed a mixed assembly method (Mix-assembly), the core of which is to cluster the genes obtained by the two methods to obtain a mixed non-redundant gene s.

Further reanalysis of Baltic environmental samples using the method resulted in a 10-fold expansion of the original gene s.

In conclusion, this study shows that the hybrid assembly method may be a feasible method to increase the information of metagenomic samples, which deserves attention and attemp.

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