A Methodology for Detection and Quantification of Esterase Activity
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Last Update: 2020-12-30
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Source: Internet
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Author: User
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Carboxylesterases are important enzymes for xenobiotic metabolism and are receiving increasing attention in the context ofcancer therapies. Quantification of individual carboxylesterase activity is important since protein levels do not always correlateto activity and significant interorgan, interindividual, and interspecies variations exist. Here we present a methodologyenabling the specific quantification of carboxylesterase 2 activity in a pool of other esterases. Method applicability isillustrated for the evaluation of interspecies variation and for activity assessment of transfected cell extracts. The methodologycan easily be adapted to the evaluation of other esterases upon careful selection of adequate substrates and/or specific inhibitors.
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