A new method of virus detection has been discovered in Germany: it has been increased to an average of 200,000 to 400,000 daily.

BEIJING, April 2 (Xinhua) -- According to foreign media reports, recently, the German Red Cross Blood Donation Department and the Frankfurt University Hospital Institute of Medical Virology, a joint research team developed a method, greatly improve the efficiency of detection of new coronavirus, without affecting the quality of diagnosis. Berlin,
, introduced the strictest ban sharply since the outbreak of new crown pneumonia began at 0:00 on March 23, with Berlin for the first time imposing tough measures to ban more than two people from gathering in public places and closing restaurants and cafes.
China News Agency reporter Peng Dawei, German hesse science minister Dorn einz announced that the new testing method will soon be available on a global scale.
she reported that scientists have found a way to detect multiple samples at the same time.
this would raise Germany's current daily test ingres of 40,000 to an average of 200,000 to 400,000, with "no impact on the quality of the diagnosis".
the joint research team is understood to be led by Professor Sevreed of the German Red Cross and Professor Chezek of the University Hospital of Frankfurt.
Dorne pointed out that one of the important goals of the new crown pneumonia fight is to improve detection capabilities, "only in this way, we can identify infected people as early as possible, isolate them, to protect others." "The approach developed by experts in Frankfurt is a remarkable invention that gives the optimistic belief that more people will be tested in the future, especially those working in areas such as health care, rescue services and the food industry," she said
.
", she also noted that, in general, samples of mucous membranes taken from the person's throat or nasal cavity are individually tested, and in the new method, experts place multiple samples simultaneously into a special solution, using the commonly known polymerase chain reaction technology (PCR) to detect whether the new coronavirus infection, the new coronavirus gene contained in the sample can be directly confirmed.
once the test results are negative, one can believe that all of the samples are free of new coronaviruses. In addition,
, the Hesse State Department of Science said in a statement that the invention has been patented in the United States and Europe, shared by Goethe University in Frankfurt and the German Red Cross Blood Donation Department, and through Goethe University knowledge transfer company Innovectis, the technology can be made available immediately to other interested institutions.
from 200 samples per day to 10,000! Wuhan new crown pneumonia virus detection why "speed up" 50 times " I must go in, I am doing virus testing.
", "This is what Lin Jia said when the traffic police blocked Lin Jia back to Wuhan.
the day of Wuhan City, Wuhan Huada Medical Inspection Institute Co., Ltd. (hereinafter referred to as "Wuhan Huada") in charge of a phone call, called back the company has returned to the company's 20 colleagues. Lin Jia, of the
Technology Department, was one of the recalled, driving back to the city alone from her home, out of line with the traffic that was queuing out of town.
traffic police told her that if you went in, you couldn't come out, and Lin Jia replied, I had no intention of coming out again.
According to the "China Times" reporter understand that during the outbreak, the third-party medical inspection agency staff have rushed to Help Wuhan, in addition to medical staff, the most important group of "retrograde."
and in the "leap" of testing ability, third-party medical institutions have also played a huge role.
nucleic acid testing is the gold standard for the diagnosis of new coronavirus infection pneumonia.
, the efficiency of the kit production and testing is critical.
early January, in Wuhan, there have been kits difficult to find, the long time to send for inspection and other issues.
Wuhan Municipal Health Committee said, before January 16, the sample needs to be sent to Beijing designated testing facilities for virus isolation and nucleic acid testing, the results return about 3-5 days;
However, this is far from meeting Wuhan's huge demand for nucleic acid testing.
January 28, the National Health and Care Commission said that Wuhan City has 59 hospitals with fever clinics, the daily number of visits has exceeded 10,000 people;
pressure, the third-party medical inspection institutions into the official field of view, has become the detection of "speed up" the backbone of the force. On January 27,
, Hubei Provincial Health and Health Commission said it would increase the number of third-party medical inspection agencies to carry out testing of nucleic acid sins of the new coronavirus.
in the list of subsequent publicity, Wuhan Huada, Wuhan Golden Domain Medical Testing Laboratory (hereinafter referred to as "Wuhan Golden Domain"), Wuhan Kangsanda Medical Inspection Institute Co., Ltd., Wuhan Dean Medical Testing Laboratory and other 13 third-party medical examination institutions with 18 CDC, 66 hospitals to assume the responsibility of nucleic acid testing in the province.
let nucleic acid testing speed up according to the "China Times" reporter understand that, in addition to obtaining government approval, the new crown virus sample testing in accordance with the highly pathogenic pathogenic microorganisms (class II) management.
nucleic acid testing of patient samples should be conducted in biosafety secondary laboratories, while using the personal protection of biosecurity tertiary laboratories.
testing laboratory to be qualified as a Clinical Gene Amplification Laboratory (PCR Laboratory), and personnel engaged in viral nucleic acid testing are required to hold a PCR qualification.
but the reality is that although generally tertiary hospitals will be equipped with qualified laboratories, but the equipment and personnel are limited, the test volume will not be too high. according to media reports
, the daily nucleic acid testing capacity of Tongji Hospital affiliated with Huazhong University of Science and Technology has a daily capacity of 1000 copies.
Wuhan Concord Jiangbei Hospital can complete up to 256 samples a day. In an interview with a crying voice, an inspector at Concord Hospital, affiliated with Huazhong University of Science and Technology,
, said they had tested 500 copies a day and were understaffed.
, by contrast, the group, chain, professional third-party medical examination institutions have more room for improvement.
third-party medical examination institution, also known as an independent medical laboratory, refers to the medical institution sedituated by the administrative department of health, with the independent legal personality, specializing in medical examination, which is a useful supplement to the existing medical resources.
day-to-day, they mainly undertake hospital samples for testing, but also with the hospital, the construction of laboratories, medical testing and pathological testing work.
gold domain medicine told the China Times reporter, Wuhan Jinyuan on January 31 officially began to undertake nucleic acid testing work.
daily detection volume from the initial few hundred cases to the peak of nearly 10,000 cases, cumulative detection of more than 150,000 cases, at peak detection volume more than a quarter of the testing demand in Wuhan City.
as of March 29, Jindomain Medical Group nucleic acid testing samples of nearly 2 million cases, the group's one-day production capacity of up to 70,000 cases.
Huada also said that Wuhan Huada Medical Inspection Institute team operation of Wuhan "fire eye" laboratory has 20,000 daily testing capacity. The peak sample reception during
operation was close to 16,000.
as of 24 hours on March 22nd, nearly 230,000 samples have been tested in Hubei.
day test volume of tens of thousands, which means that Wuhan Jinyuan and Wuhan Huada as the representative of the third-party medical inspection agencies, during the outbreak can cover most of the testing needs, took over a part of the local hospital and CDC "heavy burden", for the new crown pneumonia suspected cases of diagnosis and treatment, won a valuable time window.
, then, third-party medical examination institutions testing ability why why "leap"? This has nothing to do with human-driven technology improvements and operator overtime.
take the "Fire Eye" laboratory as an example, its equipped Huada zhi made automated sample preparation system MGISP-960, can greatly improve the detection speed, reduce the risk of manual operation.
at the same time, the lab's 24-hour response becomes the norm, and operators often have to stay up all night. Zhu Shida, head of the central China region of
, said: "Samples are usually sent at night, many are sent in the early hours of the morning, sometimes at three or four o'clock in the morning.
we can basically deliver the results of all the samples the day before by 8 a.m.
" it is understood that PCR nucleic acid detection is divided into front-end sampling, inactivation treatment, nucleic acid extraction, gene amplification and other steps.
one of the most dangerous and time-consuming is the nucleic acid extraction link, which requires the operator to open the sample tube, absorb the patient sample, carry out centrifugal treatment and other multi-process.
"Our operators can't leave the biosafety cabinet for up to three to five hours with their arms suspended, or even big movements, so as not to cause the spread of vapors containing the virus."
class down, the physical endurance requirements are very high.
" Goldfield Medical told the China Times.
treat the false positive, false negative problem detection ability to improve, how to control the quality? Previously, there have been public opinion questions about the nucleic acid test false positive, false negative high incidence of the problem.
, Tan Wei, deputy chairman of the Hubei Medical Imaging Professional Committee, said in an interview with the media that, according to his clinical experience, he thought the CT results were very consistent with the new crown pneumonia but the nucleic acid test negative cases accounted for about 30%-40%.
Sino-Japanese Friendship Hospital has also revealed that a confirmed patient admitted to the hospital, in the first three nasopharyngeal swabs new crown virus nucleic acid test are negative, until after admission to the tube on the ventilator, through the alveolar irrigation test only found the new coronavirus nucleic acid positive.
according to the "China Times" reporter understand that false negative can be caused by a variety of factors, one is in the early development of the disease, the virus content is relatively small, lower than the lower detection sensitivity limit of the test method used to cause detection can not be detected;
taking sampling as an example, Huada told this reporter, because of the rapid development of the new crown virus for lower respiratory tract infection, low content of pharynx swab, the detection of reagents put forward a high sensitivity requirements.
if the sensitivity of the test reagent is low, the sampling error can easily lead to a negative detection.
February 18, Zhong Nanshan academician also pointed out that in the sampling of nucleic acid detection, such as nasopharyngeal sampling, due to the large differences in operation, to pay special attention to the appropriateness of sampling.
third-party medical institutions are also very cautious about the problem of false positive saucy and false negative. "For potential false negatives, hospitals need to send highly suspected case samples multiple times and strengthen sampling training to ensure that qualified samples are taken, while laboratories must complete the entire operating process in accordance with strict standard operating practices,"
said.
in order to ensure the accuracy of the results and reduce the false positive situation, the suspected positive samples will be retested with different brands of kits, and the results are consistent and then reported.
": "It is important to note that, in addition to the operation that requires specification, the quality of the kit is also important.
the quality of the kits flowing into the market is uneven before the State Drug Administration does not have emergency approval registration.
some of the kits are not sensitive enough, which also results in a higher percentage of leakage.
third-party medical examination will welcome the spring? According to reporters understand that the outbreak of the early stage, patients will be priority sent to the "national team" testing, which led to on the one hand, the CDC and the hospital busy group turn, on the other hand, third-party medical inspection institutions are not easy to ensure that 10,000 people testing capacity, a large number of instruments and personnel can only idle.
, for example, from February 2, the week began, the number of samples it received every day basically did not exceed 2000, the detection flux increased to 10,000 copies / after, had more than 8000 cases of spare capacity, idle rate of 80%.
another third-party medical inspection agency Kang Sunda during the Spring Festival to pick up dozens of employees, temporarily paid a high price to buy testing reagents, directly free up six floors, a total of 15,000 square meters of testing area for the testing of the new coronavirus nucleic acid testing.
's management had expected 5,000 daily tests, but from late January to mid-February, they had only done hundreds of new coronavirus tests.
by the end of February, things are beginning to change.
Jindomain Medical told the China Times: "Government departments will take the initiative to find us to communicate production capacity, take the initiative to send samples to the company, on-site supervision report."
in the Chaokou Economic and Technological Development Zone in Wuhan Golden Domain compound, almost every day there are relevant departments to lead the scene of the 'war', hoping to see the test results as soon as possible.
" to March 1, Wuhan Huada sample reception peak has exceeded 14,000 cases, the daily detection capacity has been increased to 20,000 copies;
as of 24 o'clock on March 22, Wuhan's "Fire Eye" laboratory has completed more than 230,000 samples in Hubei.
", in the process of continuous communication with the government, we really feel the government's gradual recognition of third-party medical inspection institutions, " Jindomain medical side said, "third-party medical institutions with large scale of production capacity, strong technical force, quality assurance, advanced experimental facilities, cold chain logistics professional, easy and efficient concentration of resources and so on, in the patient early diagnosis, early isolation, early treatment, prevention of the spread of the epidemic and so on.
", Huada also said that the "fire eye" laboratory efficient lying, can not be separated from the government and the strong support of all sectors of society.
the strong co-ordination of Wuhan's new pneumonia prevention and control command, so that "fire eye" in the suspected case detection, high-risk population detection work, more fully play its scientific and technological advantages.
According to the China Times, compared with foreign countries, domestic third-party medical inspection institutions started late.
in the United States, nearly 40 percent of tests are done in independent laboratories, while third-party medical testing is only about 5 percent permeable in China.
2009, 2012 and 2013, the government has issued policies to encourage the development of third-party medical inspection agencies.
2013, the State Council's Opinions on Promoting the Development of Health Services encouraged social forces to organize medical institutions and to vigorously develop third-party inspection, testing and evaluation services.
But the third-party medical examiner's office really ushered in the outbreak in 2016.
that year, the National Health and Care Commission issued a report on allowing medical testing experiments.