A small number of cell transcription charts with spatial location information.

The Jing Naiyu Research Group of the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences has recently created a technical method to obtain a small number of cell transcription spectrometry spectrometry with spatial location information.
results of the study were published online in the Nature-Lab Guide.
in vivo tissue or in vitro cell line, there are differences between different cells, that is, the heterogeneity of cells.
heterogeneity of cells is of great significance to explain the occurrence of tumors, the multipotentiality and genealogy differentiation of stem cells or pregenital cells, and the interaction between stem cells and the microencourse.
recent years, single-cell sequencing technology has developed rapidly, making it possible to study heterogeneity between cells.
, however, the current method of single-cell sequencing loses the cell's original location information in the body, and for some research areas such as developmental biology, tumor biology, cell origin location information is very critical.
Under the guidance of Jing Nai-ho and Peng Guangtun, Chen Jun built a sequencing method that can obtain a small amount of cell transcription group information while retaining the cell's original location information by integrating and optimizing single-cell sequencing and laser micro-cutting technology: Geo-seq.
Geo-seq is an efficient, high-resolution spatial transcription group analysis scheme that can be used for the three-dimensional reconstruction of the transcription map as well as for the study of a small amount of tissue or cell transcription group information with a particular structure.
Using Geo-seq technology, the team mapped fine three-dimensional molecules in the middle of early embryonic intestinal movement in mice, and revealed spatial transcription group characteristics, transcription factors and signal pathway regulation networks during the establishment of mouse cell lineage blueprints.
that Geo-seq technology has been successfully applied to mouse brain development, liver oncology and human sperm development research.
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