A very sensitive gene detection system, the CD59 AL mutation detection system.

Genetic mutation is an important index to evaluate the risk of environmental factors causing cancer.
Wu Lijun of the Institute of Technical Biology and Agricultural Engineering of the Hefei Institute of Material Sciences of the Chinese Academy of Sciences used the CD59 mutation detection system to study the carcinogenic risk and mechanism of a variety of environmental factors, including radiation (British Journal of Cancer.2008), environmental persistent toxic pollutants (Environmental Science and Technology.2011).
studies show that CD59 AL mutation detection system is a very sensitive gene detection system.
however, these early studies are mainly based on two-dimensional in vitro cultured cells.
Although the two-dimensional in vitro culture cell experiment is simple and convenient, the results of the experiment are often quite different from the in vivo experiment.
In order to reflect the toxicity effect sofkind effect of environmental factors more realistically, the team constructed a new geno-toxictoxicity detection system of three-dimensional (3D) cells based on the AL two-dimensional mutation detection model, which was used for the genetic toxicity detection of environmental factors. first,
, 3D cell groups were obtained by low-adhesive culture method, the characteristics of 3D tissue were identified by cell morphology, dry genes and connected gene expression, and the culture conditions were optimized, and 3D cell groups with relatively stable sizes were obtained; The sudden-onset effects of nano-silver and arsenic, environmental pollutants, showed that the mutation rate of nano-silver and arsenic was much lower than that obtained in two-dimensional cells, with 99 to 7 and 70 x 3 mutations per 105 three-dimensional cells, respectively, similar to the results of animal experiments.
through ICP-MS testing found that the presence of multi-layered structures of tissues reduced the exposure of nanosilver and arsenic to internal cells, thus demonstrating resistance to nano-silver and arsenic.
the study shows that 3D cells respond more realistically to the genotoxicity of environmental factors in the body than two-dimensional cells.
results have been published online by the American Chemical Society journal Chemical Research in Toxicology (DOI: 10.1021/acs.chemrestox.8b00069).
the research is funded by the Chinese Academy of Sciences, such as Special B, the National Major Research Program, the National Natural Science Foundation and other topics.
.