Achyranthes bidentata polypeptide may induce PC12 cells to neurons through ERK1 / 2 pathway

Recently, researchers from Jiangsu Key Laboratory of nerve regeneration published a paper to study the effect of Achyranthes bidentata peptide (ABPP) on the differentiation of PC12 cells into neurons, and preliminarily explore the signal transduction pathway of ABPP on PC12 cells It is suggested that ABPP can induce neuronal differentiation of PC12 cells, which may be achieved by ERK1 / 2 signal transduction pathway This paper was published in the 12th issue of Journal of Nanjing Medical University (NATURAL SCIENCE EDITION) in 2013 PC12 cells cultured in vitro were used as the model to observe the morphological changes of PC12 cells induced by ABPP (0.25, 0.50, 1.00 μ g / ml) at different concentrations under the condition of low serum Immunofluorescence cytochemistry was used to observe the expression of neurofilament protein (NF-H) in PC12 cells induced by ABPP Western blot was used to observe the time interval (0,6,12h and 1,2,3,7d) and the concentration of ABPP (0.25,0.50,1.00 μ g / ml) after ABPP (1.0 μ g / ml) was added At the same time, PC12 cells were co cultured by ERK1 / 2 specific antagonist PD98059 and ABPP to analyze the relationship between the effect of ABPP on PC12 cells and ERK1 / 2 pathway After ABPP treatment for 3 days, some PC12 cells began to show neuron like morphology The number of neuron like cells increased gradually with the prolongation of the administration time At 7 days, the neurites of PC12 cells treated with nerve growth factor (NGF) and ABPP increased significantly, forming networks; at 14 days, the phenomenon became more and more significant On the 7th and 14th day after the administration of ABPP, the cell differentiation rate and the length of cell process were significantly increased in each concentration group, and there was a significant dose-response relationship On the 7th and 14th day, the PC12 cells in ABPP high dose group and NGF group showed NF-H positive differentiation There was a significant dose-response relationship between the activation of ERK1 / 2 by ABPP, with 1.0 μ g / ml for 2 days When PD98059 was used to inhibit the activation of ERK1 / 2, the activation of ERK1 / 2 by ABPP was partially blocked.