AcrylamideA Cysteine Alkylating Reagent for Quantitative Proteomics
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Last Update: 2021-01-04
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Source: Internet
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Author: User
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Mass spectrometry-based relative quantification of proteins is often achieved by the labeling of two samples with isotopicallylight and heavy reagents. The intensities of the ions with different masses, but same chemical properties, can be reliablyused for determining relative quantities. Several strategies of labeling with various weakness and strength and degrees ofcomplexity have been described. In this chapter, we describe a simple and inexpensive protein-labeling procedure based onthe use of acrylamide and deuterated acrylamide as a cysteine alkylating reagent. Gel electrophoresis is one of the most commonlyused techniques for analyzing/visualizing proteins, thus, we emphasize the use of acrylamide as a labeling procedure for quantifyingproteins isolated by one- and two-dimensional polyacrylamide gel electrophoresis.
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