Advances in biological preparation of the bird's nest alkyl.

The compound is a class of natural compounds widely distributed in plants, fungi, bacteria and marine life, rich in structure and with good biological activity, including the clinical treatment of cancer drug Taxol;
the important medical value of the compound, the research of the dythyme cyclolycase responsible for its biosynthetics has attracted great attention in recent years.
Bird's Nest is a class of natural products produced mainly by Cyathus, Hericium, Sarcodon, etc., with a rare characteristic 5/6/7 tricyclycical skeleton and a variety of biological activities, including antibacterial, anti-tumor, and neurogrowth factor growth.
A of the disease has therapeutic effects on model mice with Alzheimer's disease and is expected to be developed as a drug to treat Alzheimer's disease.
chemical synthesis route of these compounds is complex and difficult to apply.
development of synthetic biology has made it possible to prepare large-scale steroids in the bird's nest, the genes and enzymes responsible for the biosynthetics of these compounds remain unclear to date.
Liu Hongwei's research team at the Institute of Microbiology of the Chinese Academy of Sciences found 20 new compounds of bird's nest alkaloids with anti-tumor activity from monkey-headed mushrooms and bird's nest fungi (Fitoterapia, 2013, 76, 45; Food Chem., 2014, 152,169; J.Biol.Chem., 2015, 290, 23563; Eur.J.Med.Chem., 2016, 111,183).
to facilitate the study of the biosynthetic mechanism of the bird's nest alkyl, the team, in collaboration with Guo Shunxing, a researcher at the Institute of Medicinal Plants of the Chinese Academy of Medical Sciences, first determined the entire genome of the monkey's head mushroom.
on this basis, gene clusters responsible for the synthesis of bird's nest alkaloids were identified in Hericium erinaceum and Cyathus striatus by bioinsynomics, metabolite spectrometry and quantitative PCR techniques; Excavations revealed that EriG's congener proteins are widely distributed in marine bacteria, streptomyces, sticky bacteria, algae and fungi and other groups, and further bioinficial analysis showed that EriG and its isoglobines belong to a new member of the UbiA isoprene transferase super family, representing a new class of diazepam cyclase; The work of the
research group solves the identification problem of the bird's nest diazole cycloencase, expands people's understanding and understanding of the biochemical function of UbiA isoprene-based transferase super-family protein, enriches the type of diazine cycloenzyme, and lays an important theoretical foundation for the biological preparation of the bird's nest diazine.
above research was published online in the German Journal of Applied Chemistry (Angew.Chem.Int.Ed.2017, DOI:10.1002/anie.201700565) in the journal Angew.Chem.Int.
, an assistant researcher at the Institute of Microbiology, is the first author of the paper.
the research work has been supported and helped by Tao Yong, Wen Tingyi and Yu Wenbing Research Group.
the work was supported by the National Natural Science Foundation of China (81673334; 21472233;), the "973" project (2014CB138304) and the Strategic Bio-Resources Services Network Program (ZSTH-016) of the Chinese Academy of Sciences.
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