After Zhejiang University 80, Guo Guoxuan: Cell mapping plan is in full swing, China's strength is getting stronger and stronger

Guide: Mapping the world's first mammalian cell map, Zhejiang University "after 80" bo-guided, scientific research "delayed" singer..Mapping the world's first mammalian cell map, Zhejiang University "after 80" bo guide, by scientific research "delayed" singer ..Independent research and development of single-cell sequencing technology, mapping the world's first mammalian cell map
Qingxiu, Juya is the author to see Professor Guo Guoxuan's first impression;has received widespread attention for publishing world-renowned research in cell magazine, a leading international journal, and the 35-year-old "post-80s" professor of Zhejiang University has received widespread attentionLess than four years afterreturned home, his team developed a fully domesticized Microwell-seq high-throughput single-cell sequencing platform, and systematically analyzed more than 400,000 cells from nearly 50 organ tissues in mice to map the world's first mammalian cell mapWhy didpublish this breakthrough in such a short period of time? Professor Guo Guoxuan summed up two words: accumulation"I've been doing single-cell histology research from Ph.Dto postdoctoral, " he saysI've been in this field for ten years now, so I'm very aware of the cutting-edge technologies and ideas in the field"
difficulties
however, Professor Guo recalled, from the subject to the publication of the paper, they actually encountered some difficulties, most of them in the construction of "Microwell-seq high-throughput single-cell sequencing platform", can not find the support of the relevant domestic biotechnology industry, including how to efficiently make microplates (Microwell Plate), how to shrink beads and primers into index beads and so onEventually, by constantly groping, the team solves these problems themselvesAdvantage
summary, "Microwell-seq high-throughput single-cell sequencing platform" has three main advantages: 1) cost, it makes the cost of analyzing a single cell significantly lower than before; It can be blown to reduce the pollution rate, 3) washing, with digestive enzymes to dissociate the cell group into a single cell suspension, the suspension may appear in the suspension of cell residue, these residues may be in the microfluidic control system to contaminate each data point, and the platform can be washed process to remove the residue, so that the data is betterimprovementswhat else can be improved for the "Microwell-seq high-throughput single-cell sequencing platform", Professor Guo guoxuan shared three points: 1) the denser hole design of the microplate, increasing the probability of cell capture;Professor Guo Guoxuan,of theIndustrialization, said that after more than ten years of development, the field of single-cell histology has entered a period of vigorous developmentSingle-cell analysis technologies range from "micro-flow control" to "micro-droplets" to "microporous plates" to "intracellular markers"In the future, there will be a significant improvement in flux, and it is expected that next year, one-time capture of 1 million cells at this level will come outon whether there are plans to bring the "Microwell-seq high-throughput single-cell sequencing platform" to market for more researchers to use, Professor Guo said that despite such ideas, there is a lack of professional and technical personnel to promote the industry"I think there needs to be a part of the people who are committed to exploring truly innovative technologies in scientific research positions, and there needs to be a part of the people who are driving the industrialization of technology," he saidIf there are graduates in my lab who are willing to start a business in the future, i will fully support single-cell histological technologyTheCell Mapping Program is in full swing and expecting more support
the Human Cell Atlas (HCA) program, known as the Human Moon Landing Program in the field of biology, officially kicked off in October 2017HCA is designed to systematically map human cells, classifying and sequencing all (approximately 37 trillion) cells in the human bodyFacebook founder Mark Zuckerberg says the HCA is an international research project comparable to the Human Genome Project 'The cell map is undoubtedly very meaningful both for disease diagnosis and quality control of "seed" cells in regenerative medicine, Professor Guo Guoxuan said In fact, following the publication of mouse cell map, the current team in human, zebrafish and other species of cell mapping has made great progress "Our team understands technology, biology and data analysis with low technology costs, high throughput and high fault tolerance," said However, we have a 'big difficulty' compared to HCA, which is very weak in funding support Now, the domestic single-cell histology is developing rapidly and the strength is getting stronger and stronger In the future, it is hoped that the national level or private capital can provide more support for the domestic study of human cell mapping, so that we can more thoroughly interpret the health and different disease sourcing of human cells in the golden age of life science, Chinese scientists should compete for more say
born in 1983, Professor Guo Guoxuan graduated from Wuhan University with a major in biotechnology, and in high school, he was actually a multidisciplinary student, in physics, mathematics, chemistry, chemistry, "national middle school studentcompetition" have won awards "The reason why I chose biology when I was a university student was in part influenced by the phrase 'the 21st century is the century of biology', " professor Guo Guoxuan recalled There are many variables in life, and the mechanisms behind it are closely related to our health and quality of life, so more people need to spend more energy to analyze it now, the 21st century has passed nearly one-and-a-half, gene editing, immunotherapy and other technologies have let us see that it is indeed the golden age of life science Professor Guo said that nearly 10 years of overseas study experience has made him deeply aware that for any nation, only to build their own motherland strong, in order to make each individual real satisfaction Young researchers need to sink and make some consistent achievements in their field of study, so that China's scientific session has a more say in the international arena , Professor Guo's team is currently enrolling graduate students and postdoctoral students Turning to the criteria for selecting students, he stressed that he very much hoped that students would have a spirit of "excellence" At present, many students in China are still the implementers of the tutor's ideas, and have not been able to control their own research topics in their own right In fact, every teacher wants their students to come up with a better idea, blue is better than blue, and only in this way, a scientific field can continue to improve