Anal Chem: Qi Huan and others developed a high-performance cellulase peptide chip

Peptide chip has many advantages, such as small size, light weight, low cost, easy to carry, high flux, automatic analysis process, fast analysis speed, and few samples, etc it is widely used in the field of life science At present, the preparation of peptide chips is often limited by the composition of peptide ligands and their density and orientation on the chip surface Recently, Qi Huan, a doctoral student in the biosensor technology team of Qingdao Institute of bioenergy and process, Chinese Academy of Sciences, took the endoglucanase I (eg I) of cellulase system as a model, and conducted biological panning with the F8 / 8 scenic phage library to obtain the specific binding eg The high-performance peptide chip was successfully prepared by using egsdprmv probe, an octapeptide ligand of I, and the symmetrical carrier of F phage, which was fixed on the surface of the chip with high density and orientation Relevant results were published online in Analytical Chemistry (original h.qi, et al., analytical chemistry 2014) The researchers found that the fluorescence signal intensity of peptide chip based on phage egsdprmv probe is more than 4 times of that of traditional peptide chip (pVII egsdprmv), and the fluorescence signal is uniform, which shows that the performance of the peptide chip prepared by the symmetrical carrier FF phage fixed peptide probe is significantly better than that of the peptide chip prepared by the traditional way In addition, the peptide chip based on the bacteriophage of FF showed high sensitivity and good reproducibility The successful development of the cellulase detection chip is of great significance to the research of bioenergy based on cellulase The above research was presided over by researcher Liu Aihua Professor Valery a Petrenko of Auburn University participated in the research and was supported by NSFC and the "Hundred Talents Program" of Chinese Academy of Sciences.