Analysis and prospect of the future development direction of the blue bacterial photosynthesis cell plant.

In the context of the shortage of resources and environmental pollution, the development of photosynthesis technology, using solar energy and carbon dioxide as raw materials for the clean production of biofuels and bio-based chemicals, has become one of the important options for sustainable development.
blue bacteria is a very promising photosynthesis microbial chassis cells, with simple structure, fast growth, genetic operation and other advantages, suitable for the development of optical drive carbon sequestration cell plant.
the microbial metabolic engineering team of Qingdao Institute of Bioenergy and Process, Chinese Academy of Sciences, is committed to the research of blue bacterial metabolism engineering and synthetic biology, guided by the development of photosynthesis biomanufacturing technology system, the systematic application of synthetic biology and metabolic engineering technology, the development of new design principles and construction strategies for blue bacterial photosynthesis cell plant, successfully opened up the photosynthesis route of energy and chemical products such as sugar, ethanol, ethylene, fatty alcohol, fatty hydrocarbons and other energy and chemical products, and published in The Energy and Energy,
recently, the team published a paper on Biotechnology Advances entitled Tailoring cyanobacteria cell for improved industrial properties, discussing a new perspective on the development of the cyanobacteria photosynthesis cell plant.
in recent years, the microbial metabolic engineering team in the process of promoting the large-scale application of cyanobacteria photosynthesis manufacturing technology found that although the ability of engineering photosynthesis synthesis of natural or non-natural metabolites of engineered algae plants under laboratory conditions can be greatly improved, its scale application has been limited at both technical and economic levels.
based on the consideration of overall cost and benefit ratio, the ideal large-scale photosynthesis biological manufacturing should be able to be carried out in the outdoor, open environment, with natural light, simple treatment of sea water and even industrial sewage and industrial waste gas as the main raw materials, the harvest and extraction of cultivated biomass and target products should also be as convenient, fast and low-cost as possible.
the reality is that in the face of high temperatures, high-gloss stress, and fluctuations in the supply of light-temperature-carbon sources under conditions of far more stringent laboratory conditions, it is difficult for the blue bacterial cell plant to maintain the stable synthesis of the target product; At the same time, the physiological suitability and robustness of the existing blue-bacterial chassis algae strains are difficult to meet the requirements of stable culture, and under the condition of industrial scale culture, the biomass harvesting and target product extraction of blue bacterial cells are extremely energy-intensive and complex, thus reducing the cost competitiveness of the entire technology chain.
in response to the above, the Microbial Metabolism Engineering Team systematically presents a new perspective on industrial process-oriented metabolic engineering focusing on the development of process-oriented blue bacteria photosynthesis plants (Industrial Process Oriented Metabolic Engineering, iPOME).
to realize the large-scale application of cyanobacteria photosynthesis biological manufacturing technology, it is necessary to improve the compatibility and adaptability of photosynthesis cell plant symtonication with industrial process and process system, especially the targeted optimization of the industrial application characteristics of cyanobacteria chassis algae strains and engineering algae strains. the
research team summarized the relevant research progress and analyzed and looked forward to the future development direction of the cyanobacteria photosynthesis plant: (1) transforming the blue bacteria to overcome the limitations of day and night alternating conditions to achieve stable and sustained cell growth and product synthesis;
review paper suggests that with the in-depth understanding of the physiological activities and metabolic mechanisms of blue bacteria, as well as the development of various high-efficiency and accurate genome editing methods, a new generation of blue bacterial photosynthesis cell plants with good industrial application characteristics will be able to successfully "customize" and promote the large-scale application of photosynthesis biological manufacturing technology.
research work has been supported by the National Natural Science Foundation of China's Distinguished Youth Fund, the NSFC Sino-German cooperative research project, the Shandong Natural Science Foundation's major basic research projects, the Chinese Academy of Sciences' key deployment projects, etc.
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