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Analysis of SUC2 Promoter Structure by Nucleosome Scanning

 Last Update: 2020-12-19
 Source: Internet
 Author: User

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Chromatin remodeling is a key mode of transcriptional regulation, and studying the nucleosome positioning at promoters is an important means to understand how genes are regulated. Nucleosome scanning is a convenient method to study nucleosome positioning. Yeast cells are converted to spheroplasts and nuclei are isolated. The nuclei are then digested by micrococcal nuclease to yield mononucleosome-sized
DNA
. Using a set of overlapping primers that cover the entire promoter, quantitative real-time
PCR
is performed using the mononucleosome DNA as the template. The nucleosome enrichment for each primer is calculated to yield a map of nucleosome occupancy across the promoter.

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