Analysis of ultrasonic extraction of soybean isoflavones

I Basic information " Plant Name " Soybean " Soybean " English name " Soybean P.E Resource Distribution: China is a large soybean producer with an annual output of more than 20 million tons, ranking fourth in the world There are currently 12 types of isoflavones found in soybeans, which are classified as free-type amylons (Aglycon) and combined with glucocoide The elements account for 2% to 3% of the total, including dye mitin, soybean molybno, and Glycitein ( Gly) Sugar is accounted for about 97-98% of the total, mainly in the form of propylene dilycel dye mlysitin, propylene dilycel soyltoma, dye wood and soybean saccharin Soy isoflavones have unique effects on a variety of diseases, including breast cancer, prostate cancer, heart disease, osteoporosis, cardiovascular disease, and women's menopausal syndrome In 2000, North China Pharmaceutical Co., Ltd held a corporate standard validation meeting on soybean isoflavones By Hebei Province and Shijiazhuang City Drug Administration and drug inspection institute and other units jointly reviewed, adopted the Chinese medicine products as the standard of the "Hebei Province soybean isoflavone enterprise standards", and formally implemented The standard strictly stipulates the technical requirements, test methods, inspection rules, marking, packaging, transportation, storage, shelf life, etc of soybean isoflavones, etc., and establishes a scientific basis for the production, inspection and market development of soybean isoflavones The introduction of this standard, to fill the domestic blank Second, extraction method 1 Adsorption resin method (the method used by Nankai University) 2 Nikkei Biotechnology reported in 2000: The DuPont team in the United States successfully cloned the isoflavone synthase gene, which is of concern as a part of soy functional production, and successfully produced isoflavones from recombinant plants 3 Another report: China's bioengineering expert Dr Jiang Haokui after many years of research, successfully extracted from soybeans, and purity of more than 95%, and applied to the country 3 Research and application of ioflavone compounds is a naturally occurring phytoestrogen, in plants their main role is antioxidants, in humans and animals such substances in addition to estrogen-like effects, there are other biological activity It is found that these compounds are abundantly present in legume crops, which is a non-nutritional component in soybeans, but have obvious health effects on human beings and have no obvious toxicity, so they have been widely paid attention to Many different kinds of plants contain phytoestrogens, but soy contains high levels of zui The literature reported that soybean isoflavones are mainly distributed in the subleaves and germs of soybean seeds, and have low skin content At present, a total of 12 kinds of isoflavones in soybeans are found, divided into free-type radon and combined sugar The compound accounts for about 2 percent to 3 percent of the total, including dye mlynesin (trihydroxyflanolavones, Genitein), soy aphflavoine (dioxyxoxifone, Daidzein) and soybean monain (Glycitein) Sugar saccharin accounts for about 97% to 98% of the total, mainly in the form of clenbutetic dysaccharide dye wood (6"-O-malonylynitin), acryl-diyl soyltoniac (6"-O-malonyl daidzin), dye wood yin (Gentin) and soybean satoin (Daizin) The study on the pharmacological effects of soy isoflavones is as follows: 1 Antioxidant effect: canary inolutin (genitein) contains 5, 7, 4 three phenolic hydroxyl, soy element contains 7, 4 diazeh hydroxyl As an oxygen supply and free radical reaction, phenolic hydroxyl reacts to produce corresponding ions or molecules, extinguishing free radicals, and ending the chain reaction of free radicals Soybean isoflavones also have a clear antioxidant effect on the whole animals, soy isoflavone extract also has significant inhibition effect on the increase of peroxidation level and the decrease of antioxidant enzyme activity in mice caused by amycin 2 Estrogen-like effects: Isoflavones are typical phytoestrogens, soy isoflavones can not only replace estrogen and ER combination to play an estrogen-like role, but also interfere with estrogen and ER combination, performance of anti-estrogen-like effects Soybean isoflavones show estrogen activity or anti-estrogen activity mainly depending on the hormone metabolism of the subjects themselves For people with high estrogen levels, such as young animals and estrogenized animals and young women, anti-estrogen activity is shown, and estrogen activity is shown in those with low estrogen levels, such as young animals, de-ovarian animals, and menopausal women The estrogen-like effects of soy isoflavones may have some preventive and therapeutic effects on many hormone-relief-related diseases in older women, such as elevated blood lipids, atherosclerosis and osteoporosis 3 Anti-cancer effect: Epidemiological studies have shown that soy as a dietary source of dye mlyson may be associated with relatively low incidence of breast, colon, prostate cancer and prostate cancer in China and Japan, and that total isoflavone levels in Japanese plasma are 7-100 times higher than in the West 4 It was found that dyemone inhibited endothelial cell growth and angiogenesis in vitro, and inhibited angiogenesis showed that isoflavones could be used as therapeutic agents for new vascular diseases with a variety of chronic diseases In recent years, the research on the physiological and health effects of soybean isoflavones has been paid much attention to, and there have been many reports on the anti-cancer, anti-aging, prevention and treatment of cardiovascular disease, and the therapeutic effect of osteoporosis, but there are still many reports that are unclear or inconsistent in their results At present, most scholars believe that future animal anti-cancer trials should be in-depth study of dyes (trihydroxy flavonoids) in soy isoflavone serotonin Its anti-cancer mechanism is still not fully clear, in-depth study has very important theoretical significance and application value If the effects of dye mitone on the transduction pathway of the receptor signal without PTK active growth factor and the downstream target area of the signal transduction of the ptK active growth factor receptor are combined, the molecular mechanism of dye mlyssin inhibited tumor cell proliferation may be further revealed For example, in the study of dye mitone inhibition of topological isoenzyme activity, dna damage was rarely observed when using dye mactin to perform IC 50 concentrations in in vitro tumor suppression tests, indicating that dyes toxycin inhibited tumor cell proliferation effects may not be related to its inhibition of topological isomease activity Therefore, further research is very important In addition, many scholars especially emphasize the use of the correct means of detection of estrogen, in the absence of high sensitivity and specific ity of the detection method, should pay attention to a variety of methods to use, in order to obtain correct and reliable results At present, the development and application of soybean isoflavones only stay in the production of raw materials and lower grade health care products In short, soybean isoflavones on tumors, cardiovascular disease and menopausal syndrome is a potential chemical control agent, coupled with China's rich soybean resources, soybean food is China's traditional food, such as the development of isoflavone-rich soybean products can be used as functional food or developed into new drugs, to reduce the incidence of tumors, cardiovascular disease and menopausal syndrome or play its therapeutic role will be of great significance Fourth, product development and production and operation company, extract enterprises At present, China's production scale zui large, quality manufacturers are North China Pharmaceuticals, the rest also have the following manufacturers: Dalian Green Peak Natural Biological Products Co., Ltd., Dalian Three Foods Co., Ltd., Shandong Province Guangrao County Vegetable Oil Factory, Shandong 3D Grease Co., Ltd., North China Pharmaceutical Co., Ltd., Zhengzhou Komei Health Food Co., Ltd., Heilongjiang Province Longjiang Soybean Food Co., Ltd., Guanghan Biochemical Products Co., Ltd Chengdu Zhongren Biochemistry Co., Ltd., Shanghai Tianeng Biohealth Products Co., Ltd., Guangxi Guilin Strength Natural Food Co., Ltd., Yat-sen City Yitong Fine Chemical Co., Ltd., Xi'an Osses Bioengineering Co., Ltd., High Post City Feida Chemical Plant, Zhuhai Chuming Development Co., Ltd., Beijing Green Jinke Biotechnology Co., Ltd., Beijing Taozheng Bioengineering Technology Co., Ltd., Tianju Group Yingkou Bohai Natural Food Co., Ltd., Yankou Bohai Natural Food Co., Ltd., Fujian Province Longhua Quan Pharmaceutical Co., Ltd Shandong Green Leaf Pharmaceutical Co., Ltd Health food manufacturer Haitian Energy Biohealth Products Co., Ltd Products: Tianneng Middle-aged Treasure Tablets (including soybean isoflavones) Shijiazhuang Rongli Pharmaceutical Technology Co., Ltd Products: Gold transfer stop capsules