Application of plant genome editing tool methods.

Sequence-specific nucleases make genome editing possible and rapidly promote the development of basic and applied biology.
since the emergence of the CRISPR-Cas9 system, it has been widely used as a genome editing tool for convertable plants.
the genomic target site with CRISPR-Cas9, resulting in a fracture of the DNA double strand.
DNA double-stranded fractures are repaired primarily through two highly conservative mechanisms, non-homologous end connections (Non-homologous end joining, NHEJ) and homologous recombination (Homologous Recombination, HR).
In the NHEJ way, broken DNA is reconnected, but often inaccurate, and the fracture location results in the insertion or deletion of a small number of nucleotides, usually resulting in gene knockout mutants;
gene function identification and feature modification through genomic directional mutations have been widely used in plants.
, however, the need for accurate genome editing in plants is extremely urgent, especially for species that are difficult to convert.
today, newly developed Cas9 variants, new RNA-guided nucleases, base editing systems, and DNA-free CRISPR-Cas9 delivery methods offer unprecedented opportunities for plant genomic engineering.
Recently, Qiu Jinlong Research Group of the Institute of Microbiology of the Chinese Academy of Sciences published an article summarizing the current situation of plant genome editing, focusing on the special challenges and opportunities presented by plant genome editing(pictured), and introducing newly developed genome editing tools, methods and their potential applications in plants.
the end of the article also looks forward to the future and future direction of plant genome editing.
article was published online recently in Nature Plants.
Qiu Jinlong Research Group Assistant Researcher Yu Kangquan is the first author, Qiu Jinlong and Gao Caixia, a researcher at the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, are co-authors of the communication.
related research has been the national genetically modified special (2016ZX08010-002), the national key research and development projects (2016 YFD0100602) Beijing Science and Technology Commission project (Z1710001517001), the Chinese Academy of Sciences Strategic Pilot Science and Technology Special (XDB11030500) and the National Natural Science Foundation (31672015) and other financial support.
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