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Channel News: First, to help users quickly find samples and adjust the focal length of high-speed imaging brought about by the first benefit is to find samples more convenient.
in the "old CCD era", where the camera's imaging speed was still slow, the experimenter had to look directly under the eyepiece in order to quickly adjust the focal plane position and look for suitable samples.
is not difficult for brightfield imaging, but some fluorescent signals may be too weak to be directly observed under the eyepiece, which can cause operational inconvenience to the experimenters.
Flash 4.0's high sensitivity allows the camera to still image the sample with low exposure time under low fluorescence, and the experimenter can directly observe the sample under the mirror on the display, adjust the position of the carrier station and the focal length of the image without "delay", and greatly reduce the fluorescence power of the excitation under reasonable circumstances, so as to minimize phototoxicity.
this feature makes it unique in some special applications.
applications such as diaphragm clamps, which require as much mechanical vibration as possible, make it possible for high-speed cameras to microscope neurons with glass electrodes on the display in real time (to avoid vibrations caused by the naked eye's observation under the eyepiece).
application conditions: non-delayed real-time display requires more than 30fps camera product model recommendations: Flash4.0 LT-/Flash4.0 L II, capture high-speed motion of biological samples some biological samples in the observation process will occur rapid displacement changes, sometimes need to record these rapid displacement samples in detail, then the camera can achieve the faster frame speed, the clearer the details of these displacements.
mouse cardiomyocyte throbbing high-speed imaging 100 frames per second original application conditions: bright field high-speed imaging only requires high-speed sCMOS camera, heart-related imaging speed is generally more than 100fps.
product model recommendations: Flash4.0 LT Plus / Flash4.0 LT Zebrafish Vascular High-Speed Fluorescence 400 frames per second Slow down 20 times application conditions: Fluorescent high-speed imaging requires high sensitivity and high-speed simultaneous sCMOS camera, fast-flowing fluorescent markers require more than 200fps to observe, and camera QE needs more than 80%.
recommended: Flash4.0 V3.