Application of the naica® Droplet Chip Digital PCR System to Quantify Insulin-Encoding DNA Methylation Levels

Guided reading

Over the past few decades, the incidence of diabetes has grown significantly globally

Researchers from the State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University published a paper entitled "Prenatal exposure to a mixture of PAHs causes the dysfunction of islet cells in adult male mice: Association with type 1 in Ecotoxicology and Environmental Safety.

Application highlights:

▶ Quantification of insulin-encoding gene promoter methylation levels using the naica ^®

▶ In mice prenatally exposed to 500µg/kg PAHs, methylation levels of the insulin-encoding gene promoter were significantly increased

▶ Prenatal exposure to PAHs may contribute to the development of type 1 diabetes

The authors conducted experiments using a mixture of eight PAHs to investigate the effects of prenatal PAHs on islet cell function and quality in adulthood, while attempting to elucidate the environmental causes of type 1 diabetes onset

Research results:

▲Figure 1.

In the present study, DNA methylation levels in the insulin-encoding gene promoter were significantly increased in mouse pancreatic islets exposed to 500 µg/kg PAHs in utero, while insulin-encoding gene transcription was significantly downregulated

▲Figure 2.

The original link is as follows:introduction:

Ecotoxicology and Environmental Safety was founded in 1977 and is part of Elsevier Publishing Group

naica ^® Six-Channel Digital PCR System

^{The naica ®} six-channel digital PCR system from Stilla Technologies, a French company , is derived from the Crystal microdroplet chip digital PCR technology.