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What is Mycoplasma? Why pay attention to mycoplasma contamination in cell culture? A worldwide problem! ! ! ! Literature published by Nature shows serious problem of mycoplasma contamination in cell research field
Ewen Callaway.
Contamination hits cell work.
Nature, 29 July 2014; doi:10.
1038/511518a How can cells be protected from mycoplasma contamination? 1.
AppliChem Mycoplasma Clearance Series
2.
AppliChem Cell Contamination Prevention Series
Ewen Callaway.
Contamination hits cell work.
Nature, 29 July 2014; doi:10.
1038/511518a How can cells be protected from mycoplasma contamination? 1.
AppliChem Mycoplasma Clearance Series
| name | main ingredient | Applicable samples | use method |
| Myco-3 (Cat. No. A5240) |
Ciprofloxacin | Adherent cells that clear most mycoplasma contamination | 100-fold dilution was added to the medium, the medium was changed every 2-3 days, and the treatment was continued for 14 days |
| Myco-1 (Cat. No. A5222) |
Tiamulin | Adherent cells, together can resolve recalcitrant mycoplasma or mycoplasma contamination after treatment with Myco-3 is discontinued | Add 100-fold dilution to medium for use with Myco-2 . Use Myco-1 for 4 days and then Myco-2 for 3 days, repeat 2-3 times |
| Myco-2 (Cat. No. A5233) |
minocycline |
2.
AppliChem Cell Contamination Prevention Series
The best way to effectively protect cells is to prevent the cell culture environment, such as regular disinfection of the working environment, strict experimental operations, and ensuring the sterility of utensils
.
In the laboratory, CO2 incubators, CO2 incubator water trays, and water baths are very important sources of pollution
.
AppliChem has developed a series of pollution prevention products specifically for these 3 major pollution sources, which can effectively prevent fungi (and spores), bacteria, viruses and other pollution
.
Easy to use, non-corrosive, safe and efficient
.
.
In the laboratory, CO2 incubators, CO2 incubator water trays, and water baths are very important sources of pollution
.
AppliChem has developed a series of pollution prevention products specifically for these 3 major pollution sources, which can effectively prevent fungi (and spores), bacteria, viruses and other pollution
.
Easy to use, non-corrosive, safe and efficient
.
| name | Applicable environment | microbe prevention | use method |
| Incubator-Clean (Cat. No. A5230) |
CO2 incubators, work stations, etc. |
Mycoplasma, fungi and spores, bacteria, viruses (including HIV, HBV, etc. ) |
Ready-to-use, spray treatment can be completely wet, no need to transfer cells, it is recommended to treat every 2 weeks |
| Incuwater-Clean (Cat. No. A5219) |
C02 Incubator Water Tray | Mycoplasma, fungi and spores, bacteria, viruses (including HIV, HBV, etc. ) |
100-fold dilution into the water tray sterile water, it is recommended to replace the sterile water every 2-4 weeks |
| Aquabator-Clean (Cat. No. A9390) |
Water bath | bacteria, fungi | 100-fold dilution into the water bath, it is recommended to add it to the water bath every 1-2 weeks, and change the water to the water bath every 4-6 weeks |
3.
Applichem Mycoplasma Detection Series
Applichem Mycoplasma Detection Series
| Product number | Detection method | Applicable instruments | Kit Components |
| PCR Mycoplasma Test Kit (Cat. No. A3744) |
Ordinary PCR-electrophoresis detection |
General PCR instrument |
Reaction Mix Buffer Solution Positive Template Control |
qPCR Mycoplasma Test Kit (Cat. No. A9019) |
real-time PCR |
LightCycler®1. 2, 1. 5, 2. 0, 480, Rotor-Gene™ 3000, 6000, ABI Prism® 7000, iCycler iQ®, iQ™5, Opticon 2, Chromo 4, MX300P®, MX4000®. |
Primer/Probe/Nucleotide Mix(RED) Rehydration Buffer(BLUE) Positive Control DNA(GREEN) Internal Control DNA(YELLOW) PCR Grade Water(WHITE) |
