Approaches to Enhancing the Sensitivity of Carbohydrate Separations in Capillary Electrophoresis
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Last Update: 2020-12-23
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Source: Internet
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Author: User
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Electrophoresis in both capillaries (CE) and microchips (ME) is an extremely powerful liquid phase-separation technique thatis indispensable for the separation of carbohydrates. It is capable of separating both small mono- and disaccharides, throughto more complex oligo- and polysaccharides, with high resolution, but as with all CE and ME separations, the detection limitsare often inferior to those that can be achieved with liquid chromatographic methods. One avenue to address this is to usean on-line concentration strategy. Various approaches have been developed over the past 20 years, and this chapter will highlighttheir application to improve the sensitivity of carbohydrate separations in both CE and ME.
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