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Bacterial Transformation (Kushner Method)

 Last Update: 2020-11-23
 Source: Internet
 Author: User

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standard procedure

calcium chloride sds

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The principle of this procedure is very similar to that of the standard calcium chloride method, (
see
Chapter 34 ) i.e., exponential phase cells are harvested and washed with a solution containing divalent cations. This renders the cells
competent
, which simply means they are now able to take up
DNA
from the solution. After mixing the competent cells with the DNA, they are subjected to a heat shock to promote the uptake of DNA, presumably by affecting the physical state of the lipids in the membrane.

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