"Barcode" technology successfully traces single cell lineage history and gene expression profile

Medical Network May 20 hearing lineage tracing the history of the cell is the key to answering the fundamental problems of biology, we can learn a lot about development, aging and diseaseRecently, the Boston Children's Hospital and the Dana - Farber Cancer Institute, scientists in stem cell research program at Harvard Medical School developed a new technology --CRISPR array repair lineage-tracing (CARLIN), the so-called "bar code" technologyEdited by CRISPR genetic transformation of mouse cell line, CARLIN mouse can track the history of a single cell lineage and gene expression profiles, relevant research results were published in "Cell" magazinedecades, many developmental biologists dream is to have a method to be established with the development of the embryo or tissue, reconstruction of each cell by cell lineage - tracking the entire process from embryonic development to the adult, to reveal from the parental cell lineage of daughter cells every generation of "family tree", and how each cell is opened or closed with the passage of time which genesago, scientists only a dye or fluorescent markers to track a group of cells in miceThere are also reports the use of molecular labels or barcode bar code, but requires the information known about the markers to distinguish different cell types, but also time-consuming cell extraction and operations, which may affect their propertiesAppears CRISPR technology, researchers can make the cells barcode barcode tags without affecting cells, and lineage can track thousands of cells at the same timeResearchers now designed by the method of CARLIN a mouse model can be generated in a manner to induce transcription barcode recognition on any point in time, or adult mouse development - up to 44,000 kind of bar code, bar code encoded with the sequencing and compatible with full genetic definitionReuse of single cell RNA sequencing the gene expression pattern can be acquired for each barcode labeled cells, and then can obtain information about the type and function of the cell typesas a test case, the new system researchers reveal unknown aspects of blood development during embryogenesis, blood supplement and dynamic adult mice was observed after chemotherapyResearchers believe that their systems may also be used to understand the changes and the aging processtree lineage cellsFurthermore, the reaction system may be used to record environmental stimuli, such as pathogens, exposure and nutritional intakeIn general, the mammalian tissue to create a single-cell lineage chart is a landmark, in addition to a number of applications for research in developmental biology outside, the model will also provide important insights to understand the reaction of the organism with injuries and diseases of the affected cell types and hierarchies.