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On March 26th, the project of "Bio-High-Throughput Detection and Analysis Service Network" was jointly undertaken by the Qingdao Institute of Bioenergy and Process of the Chinese Academy of Sciences, the Beijing Institute of Life Science, Tianjin Institute of Industrial Biotechnology and the Institute of Microbiology, supported by the Science and Technology Service Network Program (STS Program) of the Chinese Academy of Sciences.
after 5 years of implementation, the project developed a single-cell Raman deselector engineering prototype (RACS-Mini), developed a single-cell Raman phenotype database and Raman group data analysis software, established a complete single-cell Raman imaging-sorting-sequencing single-cell group analysis platform, formed the first set of domestic and foreign high-throughput single-cell detection, analysis, sorting system based on Raman spectroscope, to provide new tools for life science research.
RACS-Mini is suitable for bacteria, fungi, microalgae, plant cells, human cells and other cell types, can achieve non-destructive, non-labeled conditions of 100,000 single-cell detection flux per hour, accuracy of 98.3%, after the separation of cells more than 93% of cells to maintain activity and proliferation. Based on this set of technology platforms,
has cooperated with Procter and Gamble Corporation, National Development and Investment Group Co., Ltd., Tsingtao Brewery Co., Ltd. and several hospitals in the fields of pharmaceutical health, bioenergy, industrial biotechnology, marine ecology, etc., and is building a precision oral health innovation research and transformation center with the University of Hong Kong based on single-cell detection and big data technology.
at the same time the project developed a successful microfluidic control droplet sleavirus selector prototype, the realization of laser excitation fluorescent droplet microfluidic slealike control high-throughput screening, with 7 million per hour single droplet detection flux, 2 million sorting flux, accuracy of more than 99.9%, and can achieve four-channel fluorescence detection;
the Chinese Academy of Sciences Science and Technology for Development Bureau organized the project acceptance meeting, acceptance experts agreed that the project not only in the core testing equipment and technology has significant innovation, while the project team to use the developed equipment and technology, for a number of units at home and abroad to provide technical services, resulting in positive industry impact and considerable economic benefits, suggested that the project team should further play team strength, focus on technology industrialization promotion, promote greater application results, enhance scientific and technological service capabilities.
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