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With the advent of winter, the world is facing a second wave of new crown pneumonia (COVID-19) pandemic, simple and rapid nucleic acid detection means for epidemic prevention and control is very important.
most clinically used testing technology is real-time quantitative RT-PCR technology, but the technology requires expensive equipment, high detection costs, long time.
October 26, 2020, Fudan University Wang Yongming Team, Yan Ting Team and Zheng Wei Team jointly published a paper entitled: opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-cov-2 detection online in Biosensors and Bioelectronics magazine.
research team has developed a new coronavirus detection system called "opvCRISPR", which has the characteristics of short detection time, high sensitivity and visualization of results.
integrates reverse transcriptional cyclo-mediated iso-temperature amplification technology (RT-LAMP) and Cas12a shearing into a single system that detects samples of nearly a single molecule in 45 minutes, with diagnostic results consistent with real-time quantitative RT-PCR assays.
Figure 1: opvCRISPR Detection Flow Chart 2: OpvCRISPR Method and RT-PCR Method Test Results in SARS-CoV-2 Samples Dr. Wang Rui and Dr. Qian Chunyan are co-authors of the paper, Wang Yongming Researcher, Yan Ting Researcher, Zheng Wei Researcher is the co-author of the paper.