Bunsen takes you through how to distinguish elisa experiments!

Bunsen takes you through how to distinguish elisa experiments! In recent years, ELISA kits have been widely used with the characteristics of high sensitivity and good specificity, ELISA kits are divided into several types
according to the source of the reagent and the situation of the specimen and the specific conditions of detection.

The following is explained by bunsen biology: So, which experiments are applicable to these types? 1.
Double antibody sandwich method for measuring antigens: Double antibody sandwich method is a commonly used method for detecting antigens, which is suitable for determining large molecule antigens of divalent or above, but is not suitable for the determination of semi-antigens and small molecule monovalent antigens, because it cannot form two-point
sandwiches.

2.
Double antigen sandwich method to measure antibodies: the reaction mode is similar
to that of double antibody sandwich method.

Coating and preparation of enzyme conjugates with specific antigens to detect the corresponding antibodies
.

The difference from indirect antibodies is that the enzyme label antigen is used instead of the microplate label antianti antibody
.

The specimens tested in this method do not need to be diluted and can be used directly for assays, so their sensitivity is relatively higher than that of indirect methods
.

The detection of anti-HBs in hepatitis B markers is often used in this method
.

The key to this method lies in the preparation of microplate epitapsy antigens, and a suitable labeling method should be found according to the different antigen structures
.

3.
Indirect method: it is a common method for detecting antibodies, and this method is mainly used for the detection of antibodies to pathogens and the diagnosis of infectious diseases
.

4.
Competitive method to detect antibodies: When the interfering substances in the anti-raw materials are not easy to remove, or it is not easy to obtain sufficient purified antigens, this method can be used to detect specific antibodies
.

The principle is that the antibody in the specimen and a certain amount of microplate antibody compete to bind
to the solid-phase antigen.

Anti-HBe detection is generally used in this method
.

5.
Competitive method to measure antigens: small molecule antigens or semi-antibodies lack more than two sites that can be used as sandwich methods, so they cannot be measured by double antibody sandwich method, and the competitive method mode
can be used.

The principle is that the antigen in the specimen and a certain amount of epitapsy antigen competition bind
to the solid-phase antibody.

The more antigens in the specimen, the fewer the enzyme label antigens bound to the solid phase, and the lighter
the final color.

This method
is mostly used for elisa determination of small molecule hormones and drugs.

Note: The above information is for reference only!