Can peanut oil in fat culture be replaced with soybean oil when determining the lipase vitality of microorganisms?
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Last Update: 2020-11-03
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Source: Internet
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Author: User
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: When determining the
vitality
of
microorganisms,
peanut oil in
culture can be replaced with soybean oil?
: First of all, we should analyze the main components of peanut oil box soybean oil, lipase vitality determination is generally used lysic acid as a substrate. Among natural oils, the most widely distributed ddoleic acid is the number of carbon atoms of 18 oleic acid (shun 9, shun 12-doleic acid), almost all vegetable oils contain niacin, cotton oil, soybean oil, the main component is oleic acid.
is an unsaturated fatty acid containing two double bonds. The content of lyic acid in peanut oil is also between 60% and 80%, so the two can be replaced.
but especially note that you should understand the purity of soybean oil, he should be a mixture, why not use olive oil and so on, the literature a lot of use of this? Do
medium
should not need special emulsification, high temperature sterilization should be able to emulsify!
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