Cell Rep: Screening new technologies based on phase change interaction and revealing the composition of the new coronavirus high-order protein complex

How to efficiently replicate the virus developed by Li Pilong's group? How is the replication complex formed? How does the host's immune response escape? A series of viral life history events like this are inseparable from the interaction between virus-virus and virus-host protein

Although there are many techniques used to detect protein-protein interactions, only a few of them are suitable for large-scale and high-throughput analysis of protein interactions, and they are independent of small molecule regulators that interact with targeting.

On July 17, 2021, Li Pilong's research group from the School of Life Sciences, Tsinghua University published an online study entitled "Compartmentalization-Aided Interaction Screening Reveals Extensive High-Order Complexes within the SARS-CoV-2 Proteome" in Cell Reports In the paper [3], this research uses a new technology of protein interaction based on phase change to systematically analyze the interaction network between SARS-CoV-2 viral proteins from the cell level, and creatively unearth multiple potential high-order complexes It provides more efficient and fast new targets and new strategies for the intervention of new coronary diseases

Considering the difficulty of expression and purification of some proteins and the inability to restore the true interactions in cells, the researchers designed a set of phase-change based on the basis of the previous CEBIT system, which is suitable for identifying protein interactions and Qualcomm at the cell level.

Independently of virion assembly, SARS-CoV-2 replication by Nsps multisubunit replication / transcription complex (RTC) and the nucleocapsid protein N participate mediated

In summary, the research has developed a phase-change-based protein interaction and high-throughput screening system, and cleverly linked the two together to achieve "one system for multiple purposes", and with its simplicity and ease of operation , Specific and efficient advantages, using the SARS-CoV-2 coronavirus as a model, systematically studied the binary and multiple interactions between the virus-encoded proteins, in order to deepen the understanding of the pathogenic mechanism of the virus and the follow-up of new coronary pneumonia Prevention and treatment provide new ideas and entry points

Original link:

https:// Gordon, DE, et al.

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