Cell Reports describe the molecular mechanism of RAG cell periodic regulation in V(D)J rearrangement

During development, vertebrate B lymphocytes and T lymphocytes generate diverse cell surface receptors through V(D)J rearrangement to recognize foreign antigens and participate in the body's adaptive immune process

On October 12, 2021, Peking University's School of Life Sciences and Peking University-Tsinghua Life Sciences Joint Center Researcher Hu Jiazhi's research group published a research paper entitled " RAG2 abolishes RAG1 aggregation to facilitate V(D)J recombination " in Cell Reports .

The study used confocal imaging technology to observe and prove that human RAG1 protein instead of RAG2 forms aggregates in the cell nucleus through expression and endogenous fluorescent labeling methods in a variety of cell lines (Figure 1a-b); further research found , The presence of RAG2 can destroy the condensed state of RAG1 (Figure 1c)

Figure 1.

The researchers also explored the universality of different vertebrate RAG2 proteins for the regulation of human RAG1 aggregates from an evolutionary perspective

Figure 2.

In summary, the study discovered the aggregation phenomenon of RAG1 in human lymphocytes and the mechanism of RAG2 involved in the regulation of RAG complex activity.

Figure 3.

Hu Jiazhi is the corresponding author of the paper

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