Centrifuges in RNA extraction.
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Last Update: 2020-10-27
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Source: Internet
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Author: User
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Ask: Dear comrades, would like to ask, RNA can be used in the process of RNA centrifuge, why many have to use frozen centrifuges, frozen centrifuges for what purpose ah, because there is no frozen
centrifuge
, want to use room temperature centrifuges, want to ask this has an impact on RNA?
: Room temperature centrifugation accelerates RNA degradation, and frozen centrifugation is designed to reduce the rate of RNA degradation, as is the same reason that samples are stored in an -80-degree refrigerator and liquid nitrogen grinding, both to minimize the rate of RNA degradation.
: Freezing is to reduce RNA degradation. But it feels like the requirements for RNA
not
if you do RT-PCR. We also do centrifuges at room temperature, but it doesn't affect the effect of RT. Doing other experiments is another matter.
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