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Chemical Mismatch Cleavage Analysis by Capillary Electrophoresis with Laser-Induced Fluorescence Detection

 Last Update: 2020-12-03
 Source: Internet
 Author: User

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The chemical mismatch cleavage (CMC) analysis was first described by Cotton et al. (
1
) and has successfully been used for detection and identification of mutations in several genes implicated in causing human genetic disorders (
2
-
7
). Compared with some current methods, such as denaturing gradient gel electrophoresis (
8
) and singlestranded conformation polymorphism (SSCP) which detects polymorphism in short
DNA
fragments, CMC has a higher diagnostic sensitivity and can analyze larger DNA fragment lengths (
9
).

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