Chinese peach high-quality reference genome released

Chinese peach high-quality reference genome released

Chinese peach high-quality reference genome released Chinese peach high-quality reference genome released

Recently, the Beijing Academy of Agriculture and Forestry Sciences published a research paper in Nature Communications, which published a high-quality reference genome of Chinese peaches with a unique and rich flavor, and explained that the high-quality resources represented by peaches have a great influence on the genetics of modern peach varieties worldwide.
Contribution: It reveals the mode of sweetness convergence selection and acidity differentiation selection between the East and the West in the process of peach flavor improvement, and analyzes the functions of important genes in the accumulation of fruit organic acids and soluble sugars
.

This research provides a high-quality reference genome and abundant genetic resources, laying a foundation for further in-depth analysis of the genetic mechanism of peach fruit flavor formation and promotion of genetic improvement of important quality traits

At present, the international reference genome of peach comes from the double haploid material PLOV2-2N of the Western Lovell variety.
However, Lovell is widely used as a rootstock material in its production
.

In order to reveal the important agronomic genetic basis of the fruit, this study selected Longhua nectar, one of the representative materials of Chinese peaches, and used three generations of single-molecule sequencing, supplemented by the strategy of Hi-C mounting to carry out high-quality assembly of the Longhua nectar genome (genome size).

Researchers used peach population genomic data and analyzed the population structure combined with sugar and acid-related phenotypes to reveal the convergent selection of sweetness and the differentiation selection of acidity in the Middle East and the West during the process of peach flavor improvement, and further through the combination of effective group populations The analysis of change (MSMC) and population combined infiltration (rIBD) showed the production of excellent varieties in eastern China and their genetic contribution to modern peach varieties.
The candidate genes related to sugar and acid quality in the infiltration zone and their impact on modern peach varieties were analyzed.
Potential contribution to variety formation
.

Based on the phenotypic data of peach materials for many years, the team found that in the process of peach improvement, the selection interval of the eastern and western populations was enriched with genes related to the synthesis and transport of malic acid and citric acid, which provided information for revealing the phenotypic differentiation of the two organic acids.
Candidate genes; transient overexpression of differentially expressed candidate genes confirmed that PpALMT1 can promote the accumulation of malic acid in fruits
.

Fruit sweetness is an important determinant of peach flavor and the goal of genetic improvement
.

Based on the genome-wide GWAS analysis, the researchers discovered multiple QTLs and their genetic effects that control the content of the main sugars (sucrose, fructose, glucose and sorbitol) in the pulp, providing molecular sites for the genetic improvement of peach flavor

Through the construction of the haplotype block for controlling the main effect SNP site of fructose, combined with the gene expression analysis in the haplotype block interval, a candidate gene PpERDL16 was screened.
The expression of this gene was significantly negatively correlated with the fructose content, and its homology Proteins in Arabidopsis and apples have been shown to be related to the transport of monosaccharides (glucose) across the vacuolar membrane; transient overexpression confirmed that PpERDL16 located on the vacuolar membrane can promote the export of fructose and glucose in peach pulp; gene list Body size analysis showed that modern East-West peach breeding led to the high-frequency utilization of high-fructose gene haplotypes
.

It is worth mentioning that the Beijing Academy of Agriculture and Forestry Sciences has successively made breakthroughs in the basic research of "the edible evolution mechanism of peach fruit" and "the genetic regulation mechanism of flat peach fruit shape".
The relevant research results were published in "Nature.
Newsletter and Genome Biology; the two research results provide a solid theoretical basis for the utilization of peach wild resources and flat peach breeding
.

The Beijing Academy of Agriculture and Forestry Sciences has promoted a number of good varieties recognized by the market, such as: Lvhua No.
9, Beijing No.
24, Ruiguang, Ruipan series varieties, etc.
, and actively leveraged its location advantages to coordinate the development of agricultural science and technology in the Beijing-Tianjin-Hebei region.
The revitalization of the peach industry has played a positive role
.

The research was funded by the National Key Research and Development Program "High-quality and High-yield Major Economic Crops and Industrial Quality and Efficiency Scientific Innovation" project and the Genomic Breeding Collaborative Innovation Project of Beijing Academy of Agriculture and Forestry Sciences
.

(Source: Zhang Qingdan, China Science News)

Related paper information: https://doi.

https://doi.
org/10.
1038/s41467-021-23879-2