Chinese researchers published a review of the synthesis of nitrogen heterocycles catalyzed by microorganisms

Wu Bian's research group published a review on the synthesis of nitrogen heterocycles catalyzed by microorganisms in Biotechnology Advances

Wu Bian’s research group published a review on the synthesis of nitrogen heterocyclic rings catalyzed by microorganisms in Biotechnology Advances Wu Bian’s research group published a review on the synthesis of nitrogen heterocycles catalyzed by microorganisms in Biotechnology Advances

Carbon and nitrogen are the most abundant core life elements.
Exploring the carbon and nitrogen bonding reactions in biological systems can provide clues to major scientific issues such as the origin of life and the evolution of biological metabolic pathways and regulatory mechanisms
.

As an important product type of carbon-nitrogen bonding reaction, nitrogen heterocyclic compounds are widely involved in biological genetics and metabolism; at the same time, they are also used in many industrial fields such as medicine, agriculture and food

China Ke Xueyuan Institute of Microbiology, Wu edge researchers funded by the National Natural Science Foundation of Outstanding Youth Science Foundation began to study mechanisms of microbial carbon and nitrogen analysis and the function key enzyme of remodeling
.

In recent years, his team has made a series of original innovations in the synthesis of nitrogen heterocycles catalyzed by microorganisms

Academy of Sciences

They used a computer-designed method to reconstruct the complete binding area of ​​the enzyme active center through fine hydrogen bond regulation, thus breaking through the bottleneck of the incompatibility of non-natural substrates in the hydroamination reaction in the biological system, and successfully created an ultra-wide Spectral hydroamination platform, and from this design the lactam ring cascade reaction (Nat.
Catal.
2021, 364)
.

His team also cooperated with the team of researcher Chen Yihua of the Institute of Microbiology, by introducing the method of secondary metabolic catalytic elements, to answer the de novo synthesis mechanism of the core nitrogen heterocyclic molecule NAD in the redox process of life, which is the core metabolic pathway of life.

Recently, the team and Jiang Huifeng's team from Tianjin Institute of Industrial Biotechnology published a review paper on Biotechnology Advances, summarizing the latest progress and future trends in the field of microbial catalytic synthesis of nitrogen heterocycles
.

This review first summarizes and combs the key enzymes that form natural nitrogen heterocyclic compounds, and then analyzes the nitrogen heterocyclic ring formation reactions involving industrial enzymes such as transaminase, imine reductase, and monoamine oxidase, as well as artificially designed multiple enzymes and chemistry.

In recent years, benefiting from the technological revolution brought about by microbial genetic information big data mining, enzyme engineering and synthetic biology, the enzymatic mechanism of nitrogen heterocyclic compound synthesis has become increasingly clear, and the number of enzymes that can be used for the synthesis of nitrogen heterocyclic ring has increased significantly.
The method is no longer limited to a single enzyme-catalyzed reaction, and it begins to develop into a complex metabolic pathway
.

However, there has been no corresponding summary in the field

China Kexue Yuan Dr.
Wu Feng Jing Institute of Microbiology edge research groups and Chinese Kexue Yuan Tianjin Institute of Industrial Biotechnology Geng Wen Jiang Huifeng super post-doctoral research group for the co-first authors of the review, researcher Wu edge corresponding author
.

He wrote the review was a national R & D program focused on synthetic biology projects (2018YFA0901600), the National Natural Science Foundation of Outstanding Youth Fund project (31,870,055), China Ke Xueyuan strategic network planning Biological Resources biological resources derived library (KFJ-BRP-017-58 ), China Ke Xueyuan funding cutting-edge research program "from 0-1" original innovation project (ZDBS-LY-SM014) of

Academy of Sciences Academy of Sciences

Article link: https://doi.