Chinese scholar Nature published an article: Optical genetic engineering modular optical switch-cpLOV2

Recently, Professor Wang Junfeng from the Strong Magnetic Field Laboratory of the Hefei Institute of Physics and international scholars jointly developed a new type of circular replacement photo-oxygen voltage 2 (LOV2) to expand the functional library of genetically encoded optical switches

The LOV2 domain is a blue photosensitive light switch

In this study, the researchers designed cpLOV2 using circular permutation, a powerful protein engineering method that was previously used to evolve genetically encoded fluorescent probes and new variants of biocatalysts

Using high-resolution NMR and other technologies, the researchers proved that the structural integrity and function of cpLOV2 light-induced Jα dissociation are maintained well

cpLOV2 provides more options for the application and development of optogenetics

For effectors that need a free N-terminal to perform all their functions, cpLOV2 is a better choice

Chimeric antigen receptor (CAR) T cell therapy has become a promising immunotherapy method

In a mouse model implanted with CD19+ Raji cells, the researchers used up-conversion nanoparticles (UCNPs) to convert high tissue-penetrating near-infrared light into blue light, activate the injected optoCAR-T cells, and achieve high efficiency for lymphoma Treatment

These encouraging results indicate that optoCAR-T cells can reduce potential side effects without losing the therapeutic effect

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