Clinica Chimica Acta: Multi-rRT-PCR detection of SARS-CoV-2 RNA for molecular diagnostics of COVID-19 in clinical laboratories
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Last Update: 2020-07-12
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Source: Internet
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Author: User
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Real-time reverse transcription-polymerase chain reaction (rRT-PCR) detects SARS-CoV-2 RNA and confirms the clinical diagnosis ofCOVID-19through moleculardiagnosticslaboratoryWe have developed a multi-rRT-PCR method for detecting SARS-CoV-2 RNAMultiple rRT-PCR uses three genes: sarbecev-specific E gene, SARS-CoV-2-specific N gene, and human ABL1 gene as enderthe Cq value of the simple type and multi-heavy rRT-PCR method has a good correlationLow copy of SARS-CoV-2 RNA can be detected using the new multi-rRT-PCR method."the multiple rRT-PCR approach proposed in this study will make high sensitivity detection of SARS-CoV-2 RNA possible, reducing the reagent use, cost, and time required by clinical laboratory technicians
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