Common microbial medium formula

(1) When separating bacteria, add a concentration of 50U/m to the medium,(2) when separating the linebacteria, add 0.05% xythane to the sample, when(3) separate mold and yeast, add penicillin to the medium, and(4) separate root mold and mold Because of the mycelium of these microorganisms, the medium generally used to isolate single-purpose microorganisms containinhibited it; 1, bacterial medium; formula a beef paste agar medium; beef paste 0.3 grams, protein 1.0 grams, sodium chloride 0.5 grams; water 1000 ml; in the burningWhen(5) separate bacteria, the concentration of 50U/ml mold in the medium is added to inhibit the growth of mold and yeastWhen(6) separated the discharge bacteria, the addition of 0.05% sodium sulphate (SDS) to the sample not only inhibits the growth of bacteria, but also activates the germination of the spores of the linebacteriaThe addition of fluoroquinic acid (5mg/L) and mycoycosin (50mg/L) and penicillin (0.8mg/L) can also effectively inhibit bacteria and fungi without affecting the growth of linebacteria(7) separation of mold and yeast, the medium of penicillin, streptomycin and tetracycline each 30U/ml, can inhibit the growth of bacteria and line bacteria(8) separation of root mold and mold, because these microorganisms of mycelium easily spread into pieces, it is difficult to obtain purified colonies, usually added 0.1% sodium deoxychotate or sorbitol in the medium to prevent mycelium spread, so that the colonies grow small and tightthe medium generally used to isolate single-purpose microorganisms contains inhibitors that inhibit other microorganisms, and these specialized inhibitors are more cumbersome in small laboratories and are now available in a refined product, provided that the base medium is added directlySuch as ampicillin, mainly used to isolate hydrophilic monospores1, bacterial mediumformula one beef paste agar medium beef paste 0.3 grams, protein 1.0 grams, sodium chloride 0.5 grams, agar 1.5 grams, water 1000 mlAdd 100ml of water to the beaker, add beef paste, protein and sodium chloride, mark the outside of the beaker with crayons, and heat on a fireAfter the components in the beaker have dissolved, add the agar and stir constantly to avoid sticking to the bottomAfter the full dissolution of agar to fill the water loss, with 10% hydrochloric acid or 10% sodium hydroxide to adjust the pH to 7.2 to 7.6, divided into various test tubes, plus cotton plugs, with high pressure steam sterilization for 30 minutesformula ii Potato mediumtake 250 grams of fresh bovine heart (removing fat and blood vessels), finely chopped into mince with a knife, add 500 ml of distilled water and 5 grams of proteinMark the beaker, bring to the boil and simmer over a medium heat for 2 hoursFiltered, filtered meat drying treatment, the pH of the filter to about 7.5Add 10 ml of broth and a small amount of crushed dried bovine heart, sterilizing, spareformula three-root edifying mediumglucose 10 g calcium phosphate dipotassium 0.5 g calcium carbonate 3 grams magnesium sulfate 0.2 g yeast powder 0.4 g agar 20 g water 1000 ml 1 ml 1 mlfirst boiling agar with water to dissolve, and then add other components, stirring to dissolve, mixed, sterilized, sterilized, spare2, line-and-line bacteria mediumformula 1 starch agar medium (high-tech medium)soluble starch 2 grams potassium nitrate 0.1 g hydrogen phosphate dipotassium 0.05 g sodium chloride 0.05 g magnesium sulfate 0.0 5 g iron sulphate 0.001 g agar 2 grams water 1000 mlfirst put the starch in a beaker, with 5 ml of water into a paste, pour 95 ml of water, stir and add other drugs, so that it dissolvesMark outside the beaker, heat to boil and add agar, stirring non-stop, until the agar is completely dissolved, to fill up the water lossAdjust the pH to 7.2 to 7.4, sub-packed after sterilization, spareformula two flour agar medium flour 60g agar 20g water 1000 ml to mix the flour with water into a paste, add water to 500ml and cook on a medium of fire for 30 minutes Take another 500 ml of water, put in agar, heat and boil to dissolve, mix the two liquids, replenish the water, adjust the pH to 7.4, sub-pack, sterilize, spare 3, fungal medium formula 1 Sabouraud's medium protein 10 g calathing fat 20 g maltose 40 g water 1000 ml first put the protein, agar water, heating, stirring constantly, waiting for agar to dissolve, add 40 grams of maltose (or glucose), stir it, dissolve it, then divide, sterilize, This culture bacteria is commonly used in the cultivation of many kinds of fungi recipe two potato sugar agar medium wash the potato peeled, take 200 grams cut into small pieces, add water 1000 ml, boil for half an hour, to fill up the water Add 10 grams of agar to the filter, boil and dissolve with 20 grams of sugar (for culture mold to add sucrose, for culturey yeast to add glucose), to replenish moisture, sub-pack, sterilize, spare adjust the pH of the medium to 7.2 to 7.4, and the sugar in the formula, such as glucose, can also be used to grow line bacteria and Bacillus spores recipe three soybean sprouts medium soybean sprouts 100g agar 15 g glucose 20g water 1000 ml wash edabean sprouts, add water and boil for 30 minutes Filterwithed with gauze, the leaching is added to the agar, heated and dissolved into the sugar, stirred to dissolve it, filled the moisture to 1000 ml, divided, sterilized, spare The pH of this medium is set to 7.2 to 7.4, which can be used to grow bacteria and line bacteria formula four pea agar medium pea 80 agar 5 grams water 200 ml take 80 dried peas with water, boil for 1 hour, with gauze filtration, add agar in the filter, boil to dissolve, divide, sterilize, spare 4, edible bacteria seed medium recipe a potato - sucrose - agar medium 20% potato cooking juice 1000 ml sucrose 20 g agar 18 grams the potato washes and peeled, cut into small pieces Take the potato small pieces 200 g, add water 1000 ml, boil for 20 minutes, filter Fill the juice with water to 1000 ml, i.e 20% potato juice Add agar and sucrose to the potato sauce and bring to the boil, so that it dissolves, replenishes the water, divides, sterilizes, and reserves The pH requirements of the pH are not strict with this medium and can be not measured Formula II Integrated Potato Culture 20% Potato Cooking 1000ml Potassium Dihydro phosphate 3 g magnesium sulfate 1.5 g glucose 20 g vitamin 10 mg agar 18 g first prepared 20% potato cooking juice, method the same Add the above components to the cooking juice, heat and dissolve to fill up the moisture, adjust the pH to 6 Subpacked, sterilized, spare The medium is used to culture and preserve edible bacteria such as Reishi, Mushrooms and Mushrooms