Comparison of the content of liver glycogen in full and hungry rabbits

1. Master the principles and methods of hepatic glycogen extraction.

2. To master the principles and methods of qualitatively comparing the content of liver glycogen in rabbits that are more full and hungry.

"Experimental Principles

Glycosal is one of the important storage forms of glucose in the body. Mainly stored in liver cells and muscle cells, respectively, called hepatic glycogen and creacosacin, wherein the storage of hepatic glycogen accounted for about 5% of liver weight, myosaccharine storage accounted for about 2% to 3% of muscle weight. Although the storage of hepatic glycogen is small, it is one of the important sources of sugar in the body during metabolism.

or decomposition plays an important role in regulating blood sugar concentration. Many factors can affect the content of hepatic glycogen. If the liver glycogen increases after fullness, the liver glycogen decreases gradually when hungry.

Extracted glycogen is the fresh livertissue with quartz sand and triclosan acetic acid, when the liver tissue is fully broken, of whichprotein is precipitated by triclosan acetic acid, while the glycogen is still left in the solution. < a href"" > filterremove heteroproteins. Glycosyl is insoluble in ethanol, ethanol solution is added to the upper liquid, and glycosin is precipitated from the solution.

Glycogen is a polymercompound (molecular weight of about 4 million), slightly soluble in water, the centrifugal precipitation of glycogen dissolved in water, the solution is emulsion-like gloss, that is, glycogen solution. Glycogen is non-reductive, when iodine is red-brown, hydrolytic acid can produce reductive glucose. Part of the glycogen solution is used as a color reaction of iodine, and the other part is hydrolyzed by acid to glucose, which is tested with Benedictreagents.

used the above properties to compare satiety with hungry rabbit liver glycogen.

"Equipment and Reagents

1. Equipment homogeneous machine,centrifuge, bench scale,test tube and test tube rack,berred cup, water bath pot, white porcelain plate.

2. Reagents

(1) 5% tachloro acetate solution.

(2) 0.3% iodine.

(3) 95% ethanol.

(4) 20% NaOH solution.

(5) thick hydrochloric acid solution.

(6) Ban's qualitative reagents.

"Operation Step

1. Add an appropriate amount of 5% triclosan acetate solution to the homogenizer.

2. Kill the rabbit (hunger and satiety) quickly, remove the liver by caesarean section, quickly use filter paper to suck away the attached blood, put the hungry and full rabbit liver into the homogenous mechanism to prepare liver plasma (because after the liver is removed, the liver glycogen will break down quickly). Therefore, after killing the animal, the resulting liver must be treated with triclosal acetic acid quickly to prevent decomposition.

3. Precipitation, filtration, discarding of heteroproteins, filters were collected in beech for backup, to observe and compare the turbidity of the two solutions.

4. Take 4 ml of filtration fluid from hunger and full rabbit liver homogenizer, add 95% ethanol of equal volume, mix well, centrifuge 3min (3000r/min), compare two tubes of glycogen precipitation.

5. Carefully pour out the liquid, add 5 ml of distilled water to each tube, warm it, and dissolve until the precipitation dissolves, i.e. the glycosin solution.

6. Take test tube 3, number, press the table operation:

2ml