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NetworkHelicobacter pylori infection three test methods of comparisonHelicobacter pylori (Hp) is a class of recently recognized diseases related to gastritis, peptic ulcers and other
microorganisms
. Gastritis and ulcers are known to be common organisms. It is well known that gastritis and ulcers are common diseases, and hp growth requires a special micro-oxygen demand environment, which is more difficult to do in some grass-roots laboratories. In order to find a simple, inexpensive and easy experimental method, we tried to separate, identify and compare hp in gastric biopsy specimens with urea enzyme tests and smear lenses by candlestick, as reported below. 1 Objects and Methods 1.1 Objects 1990-04-1990-10 All patients who have endoscopy at the Inner Mongolia Medical College Affiliated Hospital, after endoscopy, are in the stomach sinuses and balls and ball clamps to take mucous membrane
tissue
two pieces, put in a sterile vial, in 30min internal examination.1.2 Method (1)
Culture
and Smears: Immediately after the specimen is collected, it is inoculated in methicillin (TMP) chocolate
culture
containing polymycin B, vancomycin, bisomycin B, cornstamide, and medicinal carbon end. After the specimen is inoculated, the tissue block is repeatedly applied to the clean slide for bacterial smear Gel dyeing. (2) Take another piece of tissue into a 40-well enzyme labeling plate hole, each hole containing urea enzyme
reager
100 sl (0.1 ml), closed with transparent adhesive cloth, placed 37 degrees C temperature box, respectively, in 1/6h, 1/3h, 1/2h, 1/4h, 12h recorded color changes. The person who turns pink is positive and the person who is inastated is negative. (3)
bio
and tolerance tests are carried out in accordance with helicobacter pyridobacteria (Hp) bio-chemical identification routine. (4) Drug sensitivity test for 30 of the cultured bacteria in accordance with the conventional paper-based drug sensitivity test.2 results254 specimens in this group, of which 165 were hp-positive for direct smears, with a positive rate of 65.0%. On the smear for the advantage of bacteria, terrain-negative, bacteria have 1 to 2 bends, X-shaped or spiral, easy to identify. Direct urea enzyme test positive 170, the positive rate of 66.9%, from the time of 10min positive accounted for 40.6%, 30min accounted for 84.1%, 4h 97.7%, 12h is 100% positive. The more hp in the smear lens test, the shorter the time it takes for the urea enzyme to test positive. All 254 cases were trained by candlestick hp, and 132 cases were isolated, with a positive rate of 52.0%. The bacteria grow slowly in TMP chocolate medium, 3d to 4d when the bacteria in the dill-shaped, diameter 0.2mm to 1mm, smooth, transparent, colorless gloss, neat edges. Bio-chemical and toned tests are below Table 2. Hp isolated from the institute is sensitive to tyrosin, tetracycline, penicillin, ampicillin, and resistant to TMP-SMZ, polymycin B and nitinic acid.three tests compared with the three methods to detect 173 hp-positive cases, the positive rate of 68.1%. There was no significant difference between urea enzyme test and mirror test results (P>0.05), and the compliance rate was 95.3%. The difference between urea enzyme test and culture detection rate was significant (P<0.01), and the compliance rate was 77.6% (Table 3,4).1 Direct urea enzyme test resultsobservation time (h) 1/6 1/3 1/2 1 24 1224Totalpositive cases 69112143 154 159 166 170 170 170/254Table 2 Hp bio-chemical properties oxidasehydrogen peroxide
urea enzymeHorse uric acid hydrolysing 1% glycine tolerance H
2
S production paper method nitrate reduction 3.5% sodium chloride tolerancegrowth temperature 25 degrees C 37 degrees C 42 degrees C Table 3 Urea enzyme test and ---- - Table 3 Urea enzyme test compared with the results of the - -162 8 132 38 -381 0 84 P>0.05, urea enzyme test and mirror X
2
s 0.22; P<0.01, ureaase test culture, X
2
s 11.79. table 4 Three test methods compared index urea enzyme test scopy culture sensitivity 98.3 95.4 76.3 difference 100.0 100.0 100.0 false positive rate 0.0 0.0 0.0 negative rate 1.7 4.7 23.7 positive prediction 100.0
100.0 100.0 negative forecast 96.4 91.0 66.4 adjustment consistency 98.7 96.6 85.7 3 Discussion