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    Home > Biochemistry News > Microbiology News > Construction of Phage Antibody Repertoires from the Blood of West Nile Virus-Infected Donors

    Construction of Phage Antibody Repertoires from the Blood of West Nile Virus-Infected Donors

    • Last Update: 2021-02-16
    • Source: Internet
    • Author: User
    Tags

    innate immune cells

    chimeric monoclonal antibody
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    A method for the construction of West Nile virus immune donor antibody repertoires is described. B cells are harvested from a suitable donor and the antibody variable genes are amplified using polymerase chain reaction (
    PCR
    ). The PCR fragments are cloned in a phage display vector to construct a repertoire that can be used in panning procedures to identify many unique monoclonal antibodies.
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.
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