CRISPR/Cas9 gRNA library screens functional genes

CRLSPR/Cas9 is an accessary immune system used by bacteria and paleontogens to resist genetic material invasions such as foreign Using THERLSPR/Cas9 technology to establish a full group mutant library of mammalian > genes or a library of gene mutants associated with certain functions, through functional screening and a collection, and subsequentamplification and in-depth sequencing analysis, to identify genes associated with screening esotypes, called CRLSPR/Cas9 gRNA library. GRNA libraries are ideal tools for drug screening or targeted screening of specific paths, and the establishment of gRNA libraries will play an important role in functional gene screening, disease mechanism research, and drug development.CRlSPR/Cas9 gRNA library screening process is divided into five steps namely, gRNA library selection, gRNA library amplification, gRNA library library lyvirus packaging, gRNA library screening, PCR amplification and NGS sequencing 1, gRNA library selection