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CYP2C19-Mediated (S)-Mephenytoin 4-Hydroxylation Assayed by High-Performance Liquid Chromatography With Radiometric Detection

 Last Update: 2020-11-24
 Source: Internet
 Author: User

Tags

100 methanol

high performance liquid chromatography

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A reverse-phase, high-performance liquid chromatography method is described for the quantification of 4′-hydroxymephenytoin formed enzymatically from
14
C-labeled (
S
)-mephenytoin following incubation with c
DNA
-expressed CYP2C19 or human liver microsomes. Analytical separation is achieved using a C
18
column developed with a gradient from 10 to 100% methanol, with detection using a scintillation detector. This method is applicable to enzymatic studies for determination of CYP2C19-catalyzed (
S
)-mephenytoin 4′-hydroxylation activity.

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