Deng Hongkui's research group from the School of Life Sciences established a cultivation system for human potential expanded pluripotent stem cells (EPS cells)

On May 21, 2021, Hongkui Deng's research group of the School of Life Sciences published a research paper entitled " Chemically Defined and Xeno-free Culture Condition for Human Extended Pluripotent Stem Cells " in Nature Communications , establishing a clear composition and non-composition in vitro The human potential of heterologous components expands the culture system of pluripotent stem cells (EPS cells), and uses this system to establish a reprogramming induction method for inducing somatic cells into heterologous human EPS cells .

How to establish high-quality pluripotent stem cells has always been the core issue of stem cell research

In order to solve this problem, Deng Hongkui's research group conducted research on the cytokines and extracellular matrix secreted by the feeder layer, and established a xeno-free EPS culture condition (xeno-free EPS culture condition, Figure 1) with a defined composition

Figure 1: Establishment of a human EPS cell culture system with definite ingredients and no heterologous origin

Deng Hongkui’s research group further systematically studied the extra-embryonic development potential of human EPS cells without heterologous culture.

Figure 2: Extra-embryonic development potential of xeno-freehEPS cells

The culture system eliminates the influence of the uncertain components of feeder cells, improves the stability of human EPS cell culture, and provides conditions for promoting the wide application of human EPS cells in differentiation, disease models and developmental biology

Professor Deng Hongkui of Peking University School of Life Sciences, Peking University Stem Cell Research Center Researcher Xu Jun and Professor Bai Yun are the co-corresponding authors of the paper.

references

Li, H.

Li, R.

Sozen, B.

Tan, T.

Wang, Q.

Yang, Y.