Detection of acetylcholinesterase on red blood cell membranes.

: The detection of acetylcholinesterase on the membrane of red blood cells, as well as the preservation of blood. Please contact me for a little information.
: The short case of red blood cell acetylcholinesterase is AChE.
AchE catalytic substrate acetylbedcholine (ACTI) is used to produce -base choline, and the blue oxidized dichlorophenol pyridine (DPIP) is reduced to a colorless
compound
, and the AChE vitality unit can be determined by monitoring changes in absorbance at 600nm.
results Were measured on TheRacly 7060 sub-analyzer in 85 cases of healthy human AChE with a reference value of 46.6-61.6U/gHb. In-batch CV2.23%-2.66%, inter-batch CV4.31%-4.78%. Sensitivity 0.015U/gHb. Conclusion This method can be continuously monitored, not disturbed by hemolysis and bilirin, and is suitable for the automatic determination of AChE in clinical laboratories.
: (a) Blood preservation
The earliest blood transfusions are immediately lost to the patient after the blood is taken from the blood provider, there is no preservation problem. Blood is now generally in stock, i.e. blood has a short period of preservation in the blood bank.
in order to enter the most effective blood. That is to say, to preserve the viability of the cell, so that it can continue to survive after input, can complete its due role, for this reason must try to solve the preservation may cause cell damage problems, such as blood containers, anticoagulants, preservation fluid and other issues, the between the two are more important.
1, red blood cell storage damage
when the blood is stored in a liquid substation, red blood cells will have a
biochemical
and structural changes, these changes collectively known as red blood cell storage damage. These injuries are the main cause of changes in the survival and function of red blood cells after blood transfusion. Red blood cells that store fatal injuries in the blood are removed by the subjects soon after input.
usually a measure of blood eligibility is to see if the red blood cells that survive 24 hours after the blood is entered can reach 70% of the input, or 70% if they are qualified.
important change in storage damage is the disappearance of ATP in red blood cells. ATP degrades into ADP and then into AMP, AMP deamine becomes IMP, and then degrades, so that
nucleic acid
can be depleted. The lack of synthetic adenine in human red blood cells can be transferred in adenine phosphate when 5-phosphate-1-caramel phosphate is present.
amSynthetic AMP and produces ATP under the help of enzymes. This causes people to add adenine and phosphoric acid to their storage, thus prolonging the survival of red blood cells. Although this view has a long history and has been applied in practice, recent reports suggest that ATP levels are not directly related, but that other changes in red blood cells shorten their lifetime.
in the early stages of storage, red blood cells can change from disk to spherical, followed by the loss of membrane lipids and proteins, as well as structural protein changes. The earliest forms are associated with a decrease in ATP and can be reversed by the recovery of ATP content, but severe deformation is irreversible and is associated with less survival of red blood cells after infusion.
there are other non-metabolic factors that can affect the stability of the cell membrane. If the current PVC blood storage bag contains DEPH components, it is beneficial to prevent the action of cell membrane deformation, but its toxic effect in the blood circulation has yet to be studied.
2, anticoagulants
(1 phosphates: the heaviest anticoagulant used in blood transfusion work islye. Thechelate with the calcium ions in the blood taken, making it inactive in the response to clotting, which is metabolized by the body after a backlog of blood transfusions. Radona basic anticoagulant stored in all anticoagulants currently used. The most commonly used is sodium tritium. In addition to anticoagulant action, it also prevents hemolysis.
(2): Heparin can be used as an anticoagulant, but lacks the ability to support the metabolism of red blood cells. In heparin, the ATP of red blood cells disappears rapidly, accompanied by other storage damage after blood transfusion, and the anticoagulant effect of heparin can also be partially neutralified by heparin inhibitors and coagulant enzymes released by blood cells.
heparin anticoagulant blood must be entered within 48 hours after blood harvesting, which used to be used to prevent hypocalcemia caused by anticoagulant blood with acid, as well as for neonatal transfusion. At present, these problems are reduced by the application of concentrated red blood cells.
3, blood preservation liquid
blood preservation fluid in addition to the necessary anticoagulant effect, but also should have the ability to protect cell survival and function. In response to this requirement, the main components in today's are radon, glucose, phosphate and adenine. According to the formula is divided into ACD and CPD two categories, the two are judged to be CPD with adenine and phosphate, so can extend the preservation period of red blood cells up to 35 days, and red blood cell oxygenation function is enhanced.
e.g. only use radon acid, its righteous gas period is only 5 days, the solution of glucose is necessary for the metabolism of red blood cells nutrients, can prolong the storage time of red blood cells, and prevent hemolysis, and can make the cell
organic
phosphorus disappear slowly, to prevent damage to red blood cell storage.
ACD liquid PH is low, is not conducive to the preservation of red blood cells, can only be kept for 21 days, and the oxygenation capacity rapidly decreased, which is its disadvantage.
the development of component blood transfusions, various components have their own adaptation conditions, such as concentrated red blood cells can be preserved with crystalline salts or collome red blood cell preservation. It can also be used for cryogenic cryoprese storage. The most appropriate storage temperature for platelets is 22 degrees Celsius (room temperature).
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