Detection of DNA Sequences Using Biotinylated Probes
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Last Update: 2021-02-28
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Source: Internet
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Author: User
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It was found (
1
) that biotinylated analogs of dUTP, which contain a biotin molecule covalently bound to the C-5 position of the pyrimidine via an allylamine linker arm, can be used as substrates for
DNA
polymerase. Figure 1 shows the structure of biotin-11-dUTP, a commonly used substrate for biotinylation by the method of nick translation (
2
). The substituted DNA molecule will have similar hybridization properties to the un-substituted DNA. After hybridization to the test DNA, the biotin probe can be located by a variety of methods, including incubation with streptavidin-peroxidase conjugate, which will be described here.Fig. 1.
Structure of biotin-11-UTP.
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